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Multiplex assay reliability and long-term intra-individual variation of serologic inflammatory biomarkers.

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Date
2017-02
Authors
McKay, Heather S
Margolick, Joseph B
Martínez-Maza, Otoniel
Lopez, Joseph
Phair, John
Rappocciolo, Giovanna
Denny, Thomas N
Magpantay, Larry I
Jacobson, Lisa P
Bream, Jay H
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Abstract
BACKGROUND: Circulating cytokines, chemokines, and soluble cytokine receptors can serve as biomarkers of inflammation and immune dysregulation. Good reliability of multiplex platforms, which allow for simultaneous, comprehensive biomarker assessment, is critical for their utility in epidemiologic studies. We examined the reliability of the Meso-Scale Discovery (MSD) platform to simultaneously quantitate 15 cytokines and chemokines and the Luminex platform (R&D Systems) to quantitate 5 soluble receptors and 2 chemokines and cytokines and evaluated long-term within-person correlation of these biomarkers. METHODS: The detectability and reliability of these assay systems were assessed using the same external controls across plates and archived sera from 250 HIV(-) men in the Multicenter AIDS Cohort Study. Using up to four visits per person from 1984 to 2009, age-adjusted intraclass correlation coefficients (ICC) of biomarkers with >80% detectability (CCL11, CXCL8, CXCL10, CCL2, CCL4, CCL13, CCL17, CXCL13, IL-10, IL-12p70, IL-6, TNF-α, BAFF, sCD14, sCD27, sgp130, sIL-2Rα, and sTNF-R2) were obtained using linear mixed models. RESULTS: Most biomarkers were detectable in 80% of control samples; IFN-γ, GM-CSF, and IL-2 were undetectable in >20% of samples. Among the HIV-uninfected men, most biomarkers showed fair to strong within-person correlation (ICC>0.40) up to 15years. The ICC for CXCL8 was good in the short term but decreased with increasing time between visits, becoming lower (ICC<0.40) after 8years. CONCLUSIONS: These multiplexed assays showed acceptable reliability for use in epidemiologic research, despite some technical variability and limitations in cytokine quantitation. Most biomarkers displayed moderate-to-excellent intra-individual variability over the long term, suggesting their utility in prospective studies investigating etiologic associations with diverse chronic conditions.
Type
Journal article
Subject
Inflammatory biomarkers
Intraclass correlation
Multiplex assay reliability
Reliability
Permalink
https://hdl.handle.net/10161/13345
Published Version (Please cite this version)
10.1016/j.cyto.2016.09.018
Publication Info
McKay, Heather S; Margolick, Joseph B; Martínez-Maza, Otoniel; Lopez, Joseph; Phair, John; Rappocciolo, Giovanna; ... Bream, Jay H (2017). Multiplex assay reliability and long-term intra-individual variation of serologic inflammatory biomarkers. Cytokine, 90. pp. 185-192. 10.1016/j.cyto.2016.09.018. Retrieved from https://hdl.handle.net/10161/13345.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Denny

Thomas Norton Denny

Professor in Medicine
Thomas N. Denny, MSc, M.Phil, is the Chief Operating Officer of the Duke Human Vaccine Institute (DHVI), Associate Dean for Duke Research and Discovery @RTP, and a Professor of Medicine in the Department of Medicine at Duke University Medical Center. He is also an Affiliate Member of the Duke Global Health Institute. Previously, he served on the Health Sector Advisory Council of the Duke University Fuquay School of Business. Prior to joining Duke, he was an Associate Professor of Pathology, Labo
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