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Biodegradable Polymersomes for the Delivery of Gemcitabine to Panc-1 Cells.

dc.contributor.author Evans, Sydney M
dc.contributor.author Frail, PR
dc.contributor.author Hammer, DA
dc.contributor.author Jenkins, WT
dc.contributor.author Katz, JS
dc.contributor.author Koch, CJ
dc.contributor.author Shah, NN
dc.contributor.author Sood, N
dc.contributor.author Therien, Michael J
dc.contributor.author Yang, XY
dc.coverage.spatial Egypt
dc.date.accessioned 2017-03-01T16:16:40Z
dc.date.available 2017-03-01T16:16:40Z
dc.date.issued 2017-03-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/26167335
dc.identifier.issn 2090-9918
dc.identifier.uri https://hdl.handle.net/10161/13734
dc.description.abstract Traditional anticancer chemotherapy often displays toxic side effects, poor bioavailability, and a low therapeutic index. Targeting and controlled release of a chemotherapeutic agent can increase drug bioavailability, mitigate undesirable side effects, and increase the therapeutic index. Here we report a polymersome-based system to deliver gemcitabine to Panc-1 cells in vitro. The polymersomes were self-assembled from a biocompatible and completely biodegradable polymer, poly(ethylene oxide)-poly(caprolactone), PEO-PCL. We showed that we can encapsulate gemcitabine within stable 200 nm vesicles with a 10% loading efficiency. These vesicles displayed a controlled release of gemcitabine with 60% release after 2 days at physiological pH. Upon treatment of Panc-1 cells in vitro, vesicles were internalized as verified with fluorescently labeled polymersomes. Clonogenic assays to determine cell survival were performed by treating Panc-1 cells with varying concentrations of unencapsulated gemcitabine (FreeGem) and polymersome-encapsulated gemcitabine (PolyGem) for 48 hours. 1 μM PolyGem was equivalent in tumor cell toxicity to 1 μM FreeGem, with a one log cell kill observed. These studies suggest that further investigation on polymersome-based drug formulations is warranted for chemotherapy of pancreatic cancer.
dc.language eng
dc.relation.ispartof J Pharm (Cairo)
dc.relation.isversionof 10.1155/2013/932797
dc.title Biodegradable Polymersomes for the Delivery of Gemcitabine to Panc-1 Cells.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/26167335
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Chemistry
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 2013


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