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Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction.

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Date
2016-03-15
Authors
Al-Emran, Hassan M
Krumkamp, Ralf
Dekker, Denise Myriam
Eibach, Daniel
Aaby, Peter
Adu-Sarkodie, Yaw
Ali, Mohammad
Rubach, Mathew P
Bjerregaard-Andersen, Morten
Crump, John A
Cruz Espinoza, Ligia Maria
Løfberg, Sandra Valborg
Gassama Sow, Amy
Hertz, Julian T
Im, Justin
Jaeger, Anna
Kabore, Leon Parfait
Konings, Frank
Meyer, Christian G
Niang, Aissatou
Pak, Gi Deok
Panzner, Ursula
Park, Se Eun
Rabezanahary, Henintsoa
Rakotozandrindrainy, Raphaël
Raminosoa, Tiana Mirana
Razafindrabe, Tsiriniaina Jean Luco
Sampo, Emmanuel
Schütt-Gerowitt, Heidi
Sarpong, Nimako
Soura, Abdramane Bassiahi
Tall, Adama
von Kalckreuth, Vera
Wierzba, Thomas F
May, Jürgen
Marks, Florian
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Abstract
Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi, 14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar, Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa could advance the knowledge of serotype distribution of Salmonella.
Type
Journal article
Subject
PCR
Salmonella spp
serotyping
sub-Saharan Africa
Adolescent
Adult
Africa South of the Sahara
Child
Child, Preschool
Female
Humans
Infant
Infant, Newborn
Male
Multiplex Polymerase Chain Reaction
Salmonella Infections
Salmonella enterica
Serotyping
Young Adult
Permalink
https://hdl.handle.net/10161/13757
Published Version (Please cite this version)
10.1093/cid/civ782
Publication Info
Al-Emran, Hassan M; Krumkamp, Ralf; Dekker, Denise Myriam; Eibach, Daniel; Aaby, Peter; Adu-Sarkodie, Yaw; ... Marks, Florian (2016). Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction. Clin Infect Dis, 62 Suppl 1. pp. S80-S82. 10.1093/cid/civ782. Retrieved from https://hdl.handle.net/10161/13757.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

John Andrew Crump

Adjunct Professor in the Department of Medicine
I am based in northern Tanzania where I am Site Leader for Duke University’s collaborative research program based at Kilimanjaro Christian Medical Centre and Director of Tanzania Operations for the Duke Global Health Institute. I oversee the design and implementation of research studies on infectious diseases, particularly febrile illness, invasive bacterial disease, HIV-associated opportunistic infections, clinical trials of antiretroviral therapy and prevention of mother-to-child tr
Hertz

Julian T Hertz

Assistant Professor of Surgery
Rubach

Matthew P. Rubach

Associate Professor of Medicine
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