Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction.
Abstract
Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority
of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed.
We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le
Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures
of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance
in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica
serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella
enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme
separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi,
14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other
Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar,
Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa
could advance the knowledge of serotype distribution of Salmonella.
Type
Journal articleSubject
PCRSalmonella spp
serotyping
sub-Saharan Africa
Adolescent
Adult
Africa South of the Sahara
Child
Child, Preschool
Female
Humans
Infant
Infant, Newborn
Male
Multiplex Polymerase Chain Reaction
Salmonella Infections
Salmonella enterica
Serotyping
Young Adult
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https://hdl.handle.net/10161/13757Published Version (Please cite this version)
10.1093/cid/civ782Publication Info
Al-Emran, Hassan M; Krumkamp, Ralf; Dekker, Denise Myriam; Eibach, Daniel; Aaby, Peter;
Adu-Sarkodie, Yaw; ... Marks, Florian (2016). Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa
by Multiplex Polymerase Chain Reaction. Clin Infect Dis, 62 Suppl 1. pp. S80-S82. 10.1093/cid/civ782. Retrieved from https://hdl.handle.net/10161/13757.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
John Andrew Crump
Adjunct Professor in the Department of Medicine
I am based in northern Tanzania where I am Site Leader for Duke University’s
collaborative research program based at Kilimanjaro Christian Medical Centre and Director
of Tanzania Operations for the Duke Global Health Institute. I oversee the design
and implementation of research studies on infectious diseases, particularly febrile
illness, invasive bacterial disease, HIV-associated opportunistic infections, clinical
trials of antiretroviral therapy and prevention of mother-to-child tr
Julian T Hertz
Assistant Professor of Surgery
Matthew P. Rubach
Associate Professor of Medicine
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