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Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus.

dc.contributor.author Banura, P
dc.contributor.author Crump, John Andrew
dc.contributor.author Fields, B
dc.contributor.author Gratz, J
dc.contributor.author Hercik, C
dc.contributor.author Houpt, E
dc.contributor.author Jacob, ST
dc.contributor.author Juma, B
dc.contributor.author Kato, C
dc.contributor.author Kersh, GJ
dc.contributor.author Kibiki, Gibson S
dc.contributor.author Liu, J
dc.contributor.author Maro, A
dc.contributor.author Massung, R
dc.contributor.author Montgomery, JM
dc.contributor.author Moore, CC
dc.contributor.author Mujaga, B
dc.contributor.author Nichol, ST
dc.contributor.author Ochieng, C
dc.contributor.author Onyango, CO
dc.contributor.author Petersen, J
dc.contributor.author Scheld, WM
dc.contributor.author Sexton, C
dc.contributor.author Ströher, U
dc.contributor.author Towner, JS
dc.contributor.author Whitmer, S
dc.contributor.author Wiersma, S
dc.coverage.spatial United States
dc.date.accessioned 2017-03-02T19:00:25Z
dc.date.available 2017-03-02T19:00:25Z
dc.date.issued 2016-01
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/26491176
dc.identifier JCM.02257-15
dc.identifier.uri https://hdl.handle.net/10161/13764
dc.description.abstract Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (Bartonella spp., Brucella spp., Coxiella burnetii, Leptospira spp., Rickettsia spp., Salmonella enterica and Salmonella enterica serovar Typhi, and Yersinia pestis), and 3 protozoa (Leishmania spp., Plasmodium spp., and Trypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.
dc.language eng
dc.relation.ispartof J Clin Microbiol
dc.relation.isversionof 10.1128/JCM.02257-15
dc.subject Adult
dc.subject Communicable Diseases
dc.subject Disease Outbreaks
dc.subject Epidemiological Monitoring
dc.subject Fever of Unknown Origin
dc.subject Humans
dc.subject Molecular Diagnostic Techniques
dc.subject Real-Time Polymerase Chain Reaction
dc.subject Reference Standards
dc.subject Sensitivity and Specificity
dc.subject Time Factors
dc.title Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/26491176
pubs.begin-page 49
pubs.end-page 58
pubs.issue 1
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Infectious Diseases
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 54
dc.identifier.eissn 1098-660X


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