Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.
Repository Usage Stats
To investigate the performance of a nucleic acid amplification test (NAAT) for the diagnosis of Mycobacterium tuberculosis bacteremia, 5-ml aliquots of blood were inoculated into bioMérieux mycobacterial (MB) bottles and incubated, and 5-ml aliquots of blood were extracted and tested by real-time PCR. Of 25 samples from patients with M. tuberculosis bacteremia, 9 (36.0%) were positive and 1 (1.5%) of 66 control samples was positive by NAAT. The NAAT shows promise, but modifications should focus on improving sensitivity.
Aged, 80 and over
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Published Version (Please cite this version)10.1128/JCM.05963-11
Publication InfoCrump, John A; Tuohy, Marion J; Morrissey, Anne B; Ramadhani, Habib O; Njau, Boniface N; Maro, Venance P; ... Procop, Gary W (2012). Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. J Clin Microbiol, 50(1). pp. 138-141. 10.1128/JCM.05963-11. Retrieved from https://hdl.handle.net/10161/13790.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
More InfoShow full item record
Adjunct Professor in the Department of Medicine
I am based in northern Tanzania where I am Site Leader for Duke University’s collaborative research program based at Kilimanjaro Christian Medical Centre and Director of Tanzania Operations for the Duke Global Health Institute. I oversee the design and implementation of research studies on infectious diseases, particularly febrile illness, invasive bacterial disease, HIV-associated opportunistic infections, clinical trials of antiretroviral therapy and prevention of mother-to-child tr
Professor of Pathology
To develop and to evaluate procedures and protocols that support, enhance, and extend the ability of the clinical laboratories to carry out effectively their primary service and teaching responsibilities as relates to detection of sepsis, antimicrobial susceptibility testing, and diagnosis of tuberculosis.
Alphabetical list of authors with Scholars@Duke profiles.