Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.
Abstract
To investigate the performance of a nucleic acid amplification test (NAAT) for the
diagnosis of Mycobacterium tuberculosis bacteremia, 5-ml aliquots of blood were inoculated
into bioMérieux mycobacterial (MB) bottles and incubated, and 5-ml aliquots of blood
were extracted and tested by real-time PCR. Of 25 samples from patients with M. tuberculosis
bacteremia, 9 (36.0%) were positive and 1 (1.5%) of 66 control samples was positive
by NAAT. The NAAT shows promise, but modifications should focus on improving sensitivity.
Type
Journal articleSubject
AdolescentAdult
Aged
Aged, 80 and over
Bacteremia
Bacteriological Techniques
Blood
Humans
Middle Aged
Mycobacterium tuberculosis
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Specimen Handling
Tuberculosis
Young Adult
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https://hdl.handle.net/10161/13790Published Version (Please cite this version)
10.1128/JCM.05963-11Publication Info
(2012). Performance of nucleic acid amplification following extraction of 5 milliliters of
whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. J Clin Microbiol, 50(1). pp. 138-141. 10.1128/JCM.05963-11. Retrieved from https://hdl.handle.net/10161/13790.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
John Andrew Crump
Adjunct Professor in the Department of Medicine
I am based in northern Tanzania where I am Site Leader for Duke University’s
collaborative research program based at Kilimanjaro Christian Medical Centre and Director
of Tanzania Operations for the Duke Global Health Institute. I oversee the design
and implementation of research studies on infectious diseases, particularly febrile
illness, invasive bacterial disease, HIV-associated opportunistic infections, clinical
trials of antiretroviral therapy and prevention of mother-to-child tr
Lyman Barth Reller
Professor of Pathology
To develop and to evaluate procedures and protocols that support, enhance, and extend
the ability of the clinical laboratories to carry out effectively their primary service
and teaching responsibilities as relates to detection of sepsis, antimicrobial susceptibility
testing, and diagnosis of tuberculosis.
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