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Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.

dc.contributor.author Crump, John Andrew
dc.contributor.author Maro, Venance P
dc.contributor.author Morrissey, AB
dc.contributor.author Njau, Boniface N
dc.contributor.author Procop, GW
dc.contributor.author Ramadhani, HO
dc.contributor.author Reller, LB
dc.contributor.author Tuohy, MJ
dc.coverage.spatial United States
dc.date.accessioned 2017-03-02T19:21:12Z
dc.date.available 2017-03-02T19:21:12Z
dc.date.issued 2012-01
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/22031703
dc.identifier JCM.05963-11
dc.identifier.uri https://hdl.handle.net/10161/13790
dc.description.abstract To investigate the performance of a nucleic acid amplification test (NAAT) for the diagnosis of Mycobacterium tuberculosis bacteremia, 5-ml aliquots of blood were inoculated into bioMérieux mycobacterial (MB) bottles and incubated, and 5-ml aliquots of blood were extracted and tested by real-time PCR. Of 25 samples from patients with M. tuberculosis bacteremia, 9 (36.0%) were positive and 1 (1.5%) of 66 control samples was positive by NAAT. The NAAT shows promise, but modifications should focus on improving sensitivity.
dc.language eng
dc.relation.ispartof J Clin Microbiol
dc.relation.isversionof 10.1128/JCM.05963-11
dc.subject Adolescent
dc.subject Adult
dc.subject Aged
dc.subject Aged, 80 and over
dc.subject Bacteremia
dc.subject Bacteriological Techniques
dc.subject Blood
dc.subject Humans
dc.subject Middle Aged
dc.subject Mycobacterium tuberculosis
dc.subject Real-Time Polymerase Chain Reaction
dc.subject Sensitivity and Specificity
dc.subject Specimen Handling
dc.subject Tuberculosis
dc.subject Young Adult
dc.title Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/22031703
pubs.begin-page 138
pubs.end-page 141
pubs.issue 1
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Infectious Diseases
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 50
dc.identifier.eissn 1098-660X


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