Skip to main content
Duke University Libraries
DukeSpace Scholarship by Duke Authors
  • Login
  • Ask
  • Menu
  • Login
  • Ask a Librarian
  • Search & Find
  • Using the Library
  • Research Support
  • Course Support
  • Libraries
  • About
View Item 
  •   DukeSpace
  • Duke Scholarly Works
  • Research and Writings
  • View Item
  •   DukeSpace
  • Duke Scholarly Works
  • Research and Writings
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Ex vivo expansion of murine and human hematopoietic stem cells.

Thumbnail
View / Download
363.6 Kb
Date
2014
Authors
Doan, Phuong L
Chute, John P
Repository Usage Stats
88
views
85
downloads
Abstract
Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine bone marrow (BM) CD34(-)ckit(+)Sca1(+)Lineage(-) (CD34(-)KSL) hematopoietic stem cells (HSCs). We also describe a method to measure functional HSC content via the competitive repopulation assay. Furthermore, we summarize methods to isolate and culture human CD34(+)CD38(-)Lineage(-) cells which are enriched for human hematopoietic stem and progenitor cells.
Type
Journal article
Subject
Animals
Antigens, CD34
Antigens, CD38
Bone Marrow Cells
Cell Culture Techniques
Cell Lineage
Cell Separation
Fetal Blood
Hematopoietic Stem Cells
Humans
Mice
Permalink
https://hdl.handle.net/10161/14159
Published Version (Please cite this version)
10.1007/978-1-4939-1133-2_14
Publication Info
Doan, Phuong L; & Chute, John P (2014). Ex vivo expansion of murine and human hematopoietic stem cells. Methods Mol Biol, 1185. pp. 211-221. 10.1007/978-1-4939-1133-2_14. Retrieved from https://hdl.handle.net/10161/14159.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
Collections
  • Research and Writings
More Info
Show full item record

Scholars@Duke

Doan

Phuong Linh Doan

Associate Professor of Medicine

Material is made available in this collection at the direction of authors according to their understanding of their rights in that material. You may download and use these materials in any manner not prohibited by copyright or other applicable law.

Rights for Collection: Research and Writings


Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info

Make Your Work Available Here

How to Deposit

Browse

All of DukeSpaceCommunities & CollectionsAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit DateThis CollectionAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit Date

My Account

LoginRegister

Statistics

View Usage Statistics
Duke University Libraries

Contact Us

411 Chapel Drive
Durham, NC 27708
(919) 660-5870
Perkins Library Service Desk

Digital Repositories at Duke

  • Report a problem with the repositories
  • About digital repositories at Duke
  • Accessibility Policy
  • Deaccession and DMCA Takedown Policy

TwitterFacebookYouTubeFlickrInstagramBlogs

Sign Up for Our Newsletter
  • Re-use & Attribution / Privacy
  • Harmful Language Statement
  • Support the Libraries
Duke University