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Ex vivo expansion of murine and human hematopoietic stem cells.

dc.contributor.author Doan, Phuong Linh
dc.contributor.author Chute, JP
dc.coverage.spatial United States
dc.date.accessioned 2017-04-28T15:08:33Z
dc.date.available 2017-04-28T15:08:33Z
dc.date.issued 2014
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/25062631
dc.identifier.uri https://hdl.handle.net/10161/14159
dc.description.abstract Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine bone marrow (BM) CD34(-)ckit(+)Sca1(+)Lineage(-) (CD34(-)KSL) hematopoietic stem cells (HSCs). We also describe a method to measure functional HSC content via the competitive repopulation assay. Furthermore, we summarize methods to isolate and culture human CD34(+)CD38(-)Lineage(-) cells which are enriched for human hematopoietic stem and progenitor cells.
dc.language eng
dc.relation.ispartof Methods Mol Biol
dc.relation.isversionof 10.1007/978-1-4939-1133-2_14
dc.subject Animals
dc.subject Antigens, CD34
dc.subject Antigens, CD38
dc.subject Bone Marrow Cells
dc.subject Cell Culture Techniques
dc.subject Cell Lineage
dc.subject Cell Separation
dc.subject Fetal Blood
dc.subject Hematopoietic Stem Cells
dc.subject Humans
dc.subject Mice
dc.title Ex vivo expansion of murine and human hematopoietic stem cells.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/25062631
pubs.begin-page 211
pubs.end-page 221
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Hematological Malignancies
pubs.organisational-group School of Medicine
pubs.publication-status Published
pubs.volume 1185
dc.identifier.eissn 1940-6029


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