| dc.contributor.author |
Doan, Phuong L |
|
| dc.contributor.author |
Chute, John P |
|
| dc.coverage.spatial |
United States |
|
| dc.date.accessioned |
2017-04-28T15:08:33Z |
|
| dc.date.available |
2017-04-28T15:08:33Z |
|
| dc.date.issued |
2014 |
|
| dc.identifier |
https://www.ncbi.nlm.nih.gov/pubmed/25062631 |
|
| dc.identifier.uri |
https://hdl.handle.net/10161/14159 |
|
| dc.description.abstract |
Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety
of the mature blood and immune system throughout the lifespan of an organism. Here,
we describe methods to isolate and culture murine bone marrow (BM) CD34(-)ckit(+)Sca1(+)Lineage(-)
(CD34(-)KSL) hematopoietic stem cells (HSCs). We also describe a method to measure
functional HSC content via the competitive repopulation assay. Furthermore, we summarize
methods to isolate and culture human CD34(+)CD38(-)Lineage(-) cells which are enriched
for human hematopoietic stem and progenitor cells.
|
|
| dc.language |
eng |
|
| dc.publisher |
Springer New York |
|
| dc.relation.ispartof |
Methods Mol Biol |
|
| dc.relation.isversionof |
10.1007/978-1-4939-1133-2_14 |
|
| dc.subject |
Animals |
|
| dc.subject |
Antigens, CD34 |
|
| dc.subject |
Antigens, CD38 |
|
| dc.subject |
Bone Marrow Cells |
|
| dc.subject |
Cell Culture Techniques |
|
| dc.subject |
Cell Lineage |
|
| dc.subject |
Cell Separation |
|
| dc.subject |
Fetal Blood |
|
| dc.subject |
Hematopoietic Stem Cells |
|
| dc.subject |
Humans |
|
| dc.subject |
Mice |
|
| dc.title |
Ex vivo expansion of murine and human hematopoietic stem cells. |
|
| dc.type |
Journal article |
|
| duke.contributor.id |
Doan, Phuong L|0110004 |
|
| pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/25062631 |
|
| pubs.begin-page |
211 |
|
| pubs.end-page |
221 |
|
| pubs.organisational-group |
Clinical Science Departments |
|
| pubs.organisational-group |
Duke |
|
| pubs.organisational-group |
Duke Cancer Institute |
|
| pubs.organisational-group |
Institutes and Centers |
|
| pubs.organisational-group |
Medicine |
|
| pubs.organisational-group |
Medicine, Hematological Malignancies |
|
| pubs.organisational-group |
School of Medicine |
|
| pubs.publication-status |
Published |
|
| pubs.volume |
1185 |
|
| dc.identifier.eissn |
1940-6029 |
|
| duke.contributor.orcid |
Doan, Phuong L|0000-0003-1361-2068 |
|
