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Laboratory accuracy improvement in the uk neqas leucocyte immunophenotyping immune monitoring program: An eleven-year review via longitudinal mixed effects modeling.

dc.contributor.author Bainbridge, John
dc.contributor.author Rountree, Wes
dc.contributor.author Louzao, Raul
dc.contributor.author Wong, John
dc.contributor.author Whitby, L
dc.contributor.author Denny, Thomas Norton
dc.contributor.author Barnett, David
dc.coverage.spatial United States
dc.date.accessioned 2017-06-01T18:30:14Z
dc.date.available 2017-06-01T18:30:14Z
dc.date.issued 2017-05-08
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/28480599
dc.identifier.uri http://hdl.handle.net/10161/14663
dc.description.abstract BACKGROUND: The United Kingdom National External Quality Assessment Service (UK NEQAS) for Leucocyte Immunophenotyping Immune Monitoring Programme, provides external quality assessment (EQA) to non-U.S. laboratories affiliated with the NIH NIAID Division of AIDS (DAIDS) clinical trials networks. Selected laboratories are required to have oversight, performance monitoring, and remediation undertaken by Immunology Quality Assessment (IQA) staff under the DAIDS contract. We examined whether laboratory accuracy improves with longer EQA participation and whether IQA remediation is effective. METHODS: Laboratory accuracy, defined by the measurement residuals from trial sample medians, was measured on four outcomes: both CD4+ absolute counts (cells/μL) and percentages; and CD8+ absolute counts (cells/μL) and percentages. Three laboratory categories were defined: IQA monitored (n = 116), United Kingdom/non-DAIDS (n = 137), and non-DAIDS/non-UK (n = 1034). For absolute count outcomes, the groups were subdivided into single platform and dual platform users. RESULTS: Increasing EQA duration was found to be associated with increasing accuracy for all groups in all four lymphocyte subsets (P < 0.0001). In the percentage outcomes, the typical IQA group laboratory improved faster than laboratories from the other two groups (P < 0.005). No difference in the overall rate of improvement was found between groups for absolute count outcomes. However, in the DPT subgroup the IQA group ultimately showed greater homogeneity. CONCLUSIONS: EQA participation coupled with effective laboratory monitoring and remedial action is strongly associated with improved laboratory accuracy, both incrementally and in the proportion of laboratories meeting suggested standards. Improvement in accuracy provides more reliable laboratory information facilitating more appropriate patient treatment decisions. © 2017 International Clinical Cytometry Society.
dc.language eng
dc.relation.ispartof Cytometry B Clin Cytom
dc.relation.isversionof 10.1002/cyto.b.21531
dc.subject flow cytometry
dc.subject mixed effects model
dc.subject quality assessment
dc.subject quality improvement
dc.title Laboratory accuracy improvement in the uk neqas leucocyte immunophenotyping immune monitoring program: An eleven-year review via longitudinal mixed effects modeling.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/28480599
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Human Vaccine Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Duke Human Vaccine Institute
pubs.organisational-group School of Medicine
pubs.organisational-group Staff
pubs.publication-status Published online
dc.identifier.eissn 1552-4957


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