Development of a contemporary globally diverse HIV viral panel by the EQAPOL program.
Abstract
The significant diversity among HIV-1 variants poses serious challenges for vaccine
development and for developing sensitive assays for screening, surveillance, diagnosis,
and clinical management. Recognizing a need to develop a panel of HIV representing
the current genetic and geographic diversity NIH/NIAID contracted the External Quality
Assurance Program Oversight Laboratory (EQAPOL) to isolate, characterize and establish
panels of HIV-1 strains representing global diverse subtypes and circulating recombinant
forms (CRFs), and to make them available to the research community. HIV-positive plasma
specimens and previously established isolates were collected through a variety of
collaborations with a preference for samples from acutely/recently infected persons.
Source specimens were cultured to high-titer/high-volume using well-characterized
cryopreserved PBMCs from National y donors. Panel samples were stored as neat culture
supernatant or diluted into defibrinated plasma. Characterization for the final expanded
virus stocks included viral load, p24 antigen, infectivity (TCID), sterility, coreceptor
usage, and near full-length genome sequencing. Viruses are made available to approved,
interested laboratories using an online ordering application. The current EQAPOL Viral
Diversity panel includes 100 viral specimens representing 6 subtypes (A, B, C, D,
F, and G), 2 sub-subtypes (F1 and F2), 7 CRFs (01, 02, 04, 14, 22, 24, and 47), 19
URFs and 3 group O viruses from 22 countries. The EQAPOL Viral Diversity panel is
an invaluable collection of well-characterized reagents that are available to the
scientific community, including researchers, epidemiologists, and commercial manufacturers
of diagnostics and pharmaceuticals to support HIV research, as well as diagnostic
and vaccine development.
Type
Journal articleSubject
EQAPOLHIV
Recombinant
Subtype panel
AIDS Vaccines
Biological Specimen Banks
Biomarkers
Cell Survival
Cells, Cultured
Cryopreservation
Cytokines
Genotype
Guideline Adherence
HIV Infections
HIV-1
Humans
Immunologic Tests
International Cooperation
Laboratory Proficiency Testing
Leukocytes, Mononuclear
Monitoring, Immunologic
Observer Variation
Phenotype
Practice Guidelines as Topic
Predictive Value of Tests
Program Development
Program Evaluation
Quality Control
Quality Indicators, Health Care
Reproducibility of Results
Specimen Handling
Time Factors
Treatment Outcome
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https://hdl.handle.net/10161/14692Published Version (Please cite this version)
10.1016/j.jim.2014.01.004Publication Info
Sanchez, Ana M; DeMarco, C Todd; Hora, Bhavna; Keinonen, Sarah; Chen, Yue; Brinkley,
Christie; ... Denny, Thomas N (2014). Development of a contemporary globally diverse HIV viral panel by the EQAPOL program.
J Immunol Methods, 409. pp. 117-130. 10.1016/j.jim.2014.01.004. Retrieved from https://hdl.handle.net/10161/14692.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Thomas Norton Denny
Professor in Medicine
Thomas N. Denny, MSc, M.Phil, is the Chief Operating Officer of the Duke Human Vaccine
Institute (DHVI), Associate Dean for Duke Research and Discovery @RTP, and a Professor
of Medicine in the Department of Medicine at Duke University Medical Center. He is
also an Affiliate Member of the Duke Global Health Institute. Previously, he served
on the Health Sector Advisory Council of the Duke University Fuquay School of Business.
Prior to joining Duke, he was an Associate Professor of Pathology, Labo
Feng Gao
Professor Emeritus in Medicine
Dr. Feng Gao is Professor of Medicine at Duke University. The Gao laboratory has a
long-standing interest in elucidating the origins and evolution of human and simian
inmmunodeficiency viruses (HIV and SIV), and in studying HIV/SIV gene function and
pathogenic mechanisms from the evolutionary perspective. These studies have led to
new strategies to better understand HIV origins, biology, pathogenesis and drug resistance,
and to design new AIDS vaccines.
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