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Development of a contemporary globally diverse HIV viral panel by the EQAPOL program.

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Date
2014-07
Authors
Sanchez, Ana M
DeMarco, C Todd
Hora, Bhavna
Keinonen, Sarah
Chen, Yue
Brinkley, Christie
Stone, Mars
Tobler, Leslie
Keating, Sheila
Schito, Marco
Busch, Michael P
Gao, Feng
Denny, Thomas N
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(13 total)
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Abstract
The significant diversity among HIV-1 variants poses serious challenges for vaccine development and for developing sensitive assays for screening, surveillance, diagnosis, and clinical management. Recognizing a need to develop a panel of HIV representing the current genetic and geographic diversity NIH/NIAID contracted the External Quality Assurance Program Oversight Laboratory (EQAPOL) to isolate, characterize and establish panels of HIV-1 strains representing global diverse subtypes and circulating recombinant forms (CRFs), and to make them available to the research community. HIV-positive plasma specimens and previously established isolates were collected through a variety of collaborations with a preference for samples from acutely/recently infected persons. Source specimens were cultured to high-titer/high-volume using well-characterized cryopreserved PBMCs from National y donors. Panel samples were stored as neat culture supernatant or diluted into defibrinated plasma. Characterization for the final expanded virus stocks included viral load, p24 antigen, infectivity (TCID), sterility, coreceptor usage, and near full-length genome sequencing. Viruses are made available to approved, interested laboratories using an online ordering application. The current EQAPOL Viral Diversity panel includes 100 viral specimens representing 6 subtypes (A, B, C, D, F, and G), 2 sub-subtypes (F1 and F2), 7 CRFs (01, 02, 04, 14, 22, 24, and 47), 19 URFs and 3 group O viruses from 22 countries. The EQAPOL Viral Diversity panel is an invaluable collection of well-characterized reagents that are available to the scientific community, including researchers, epidemiologists, and commercial manufacturers of diagnostics and pharmaceuticals to support HIV research, as well as diagnostic and vaccine development.
Type
Journal article
Subject
EQAPOL
HIV
Recombinant
Subtype panel
AIDS Vaccines
Biological Specimen Banks
Biomarkers
Cell Survival
Cells, Cultured
Cryopreservation
Cytokines
Genotype
Guideline Adherence
HIV Infections
HIV-1
Humans
Immunologic Tests
International Cooperation
Laboratory Proficiency Testing
Leukocytes, Mononuclear
Monitoring, Immunologic
Observer Variation
Phenotype
Practice Guidelines as Topic
Predictive Value of Tests
Program Development
Program Evaluation
Quality Control
Quality Indicators, Health Care
Reproducibility of Results
Specimen Handling
Time Factors
Treatment Outcome
Permalink
https://hdl.handle.net/10161/14692
Published Version (Please cite this version)
10.1016/j.jim.2014.01.004
Publication Info
Sanchez, Ana M; DeMarco, C Todd; Hora, Bhavna; Keinonen, Sarah; Chen, Yue; Brinkley, Christie; ... Denny, Thomas N (2014). Development of a contemporary globally diverse HIV viral panel by the EQAPOL program. J Immunol Methods, 409. pp. 117-130. 10.1016/j.jim.2014.01.004. Retrieved from https://hdl.handle.net/10161/14692.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Denny

Thomas Norton Denny

Professor in Medicine
Thomas N. Denny, MSc, M.Phil, is the Chief Operating Officer of the Duke Human Vaccine Institute (DHVI), Associate Dean for Duke Research and Discovery @RTP, and a Professor of Medicine in the Department of Medicine at Duke University Medical Center. He is also an Affiliate Member of the Duke Global Health Institute. Previously, he served on the Health Sector Advisory Council of the Duke University Fuquay School of Business. Prior to joining Duke, he was an Associate Professor of Pathology, Labo
Gao

Feng Gao

Professor Emeritus in Medicine
Dr. Feng Gao is Professor of Medicine at Duke University. The Gao laboratory has a long-standing interest in elucidating the origins and evolution of human and simian inmmunodeficiency viruses (HIV and SIV), and in studying HIV/SIV gene function and pathogenic mechanisms from the evolutionary perspective. These studies have led to new strategies to better understand HIV origins,  biology, pathogenesis and drug resistance, and to design new AIDS vaccines.
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