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Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials

dc.contributor.author Sambor, Anna
dc.contributor.author Garcia, Ambrosia
dc.contributor.author Berrong, Mark
dc.contributor.author Pickeral, Joy
dc.contributor.author Brown, Sara
dc.contributor.author Rountree, Wes
dc.contributor.author Sanchez, Ana
dc.contributor.author Pollara, Justin
dc.contributor.author Frahm, Nicole
dc.contributor.author Keinonen, Sarah
dc.contributor.author Kijak, Gustavo H
dc.contributor.author Roederer, Mario
dc.contributor.author Levine, Gail
dc.contributor.author D'Souza, M Patricia
dc.contributor.author Jaimes, Maria
dc.contributor.author Koup, Richard
dc.contributor.author Denny, Thomas
dc.contributor.author Cox, Josephine
dc.contributor.author Ferrari, Guido
dc.date.accessioned 2017-06-01T20:09:46Z
dc.date.available 2017-06-01T20:09:46Z
dc.date.issued 2014-01-01
dc.identifier.issn 0022-1759
dc.identifier.uri https://hdl.handle.net/10161/14714
dc.description.abstract A large repository of cryopreserved peripheral blood mononuclear cells (PBMCs) samples was created to provide laboratories testing the specimens from human immunodeficiency virus-1 (HIV-1) vaccine clinical trials the material for assay development, optimization, and validation. One hundred thirty-one PBMC samples were collected using leukapheresis procedure between 2007 and 2013 by the Comprehensive T cell Vaccine Immune Monitoring Consortium core repository. The donors included 83 human immunodeficiency virus-1 (HIV-1) seronegative and 32 HIV-1 seropositive subjects. The samples were extensively characterized for the ability of T cell subsets to respond to recall viral antigens including cytomegalovirus, Epstein-Barr virus, influenza virus, and HIV-1 using Interferon-gamma (IFN-γ) enzyme linked immunospot (ELISpot) and IFN-γ/interleukin 2 (IL-2) intracellular cytokine staining (ICS) assays. A subset of samples was evaluated over time to determine the integrity of the cryopreserved samples in relation to recovery, viability, and functionality. The principal results of our study demonstrate that viable and functional cells were consistently recovered from the cryopreserved samples. Therefore, we determined that this repository of large size cryopreserved cellular samples constitutes a unique resource for laboratories that are involved in optimization and validation of assays to evaluate T, B, and NK cellular functions in the context of clinical trials. © 2014 Elsevier B.V.
dc.publisher Elsevier BV
dc.relation.ispartof Journal of Immunological Methods
dc.relation.isversionof 10.1016/j.jim.2014.04.005
dc.title Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials
dc.type Journal article
duke.contributor.id Pollara, Justin|0300144
duke.contributor.id Denny, Thomas|0400543
pubs.begin-page 107
pubs.end-page 116
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Human Vaccine Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Duke Human Vaccine Institute
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group School of Medicine
pubs.organisational-group Surgery
pubs.organisational-group Surgery, Surgical Sciences
pubs.publication-status Published
pubs.volume 409
dc.identifier.eissn 1872-7905


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