In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature.
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We performed epi-mode pump-probe imaging of melanin in excised human pigmented lesions and both hemoglobin and melanin in live xenograft mouse melanoma models to depths greater than 100 µm. Eumelanin and pheomelanin images, which have been previously demonstrated to differentiate melanoma from benign lesions, were acquired at the dermal-epidermal junction with cellular resolution and modest optical powers (down to 15 mW). We imaged dermal microvasculature with the same wavelengths, allowing simultaneous acquisition of melanin, hemoglobin and multiphoton autofluorescence images. Molecular pump-probe imaging of melanocytes, skin structure and microvessels allows comprehensive, non-invasive characterization of pigmented lesions.
Subject(170.3880) Medical and biological imaging
(180.5810) Scanning microscopy
(320.7150) Ultrafast spectroscopy
Published Version (Please cite this version)10.1364/BOE.2.001576
Publication InfoMatthews, TE; Wilson, JW; Degan, Simone; Simpson, MJ; Jin, JY; Zhang, Jennifer; & Warren, WS (2011). In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature. Biomed Opt Express, 2(6). pp. 1576-1583. 10.1364/BOE.2.001576. Retrieved from http://hdl.handle.net/10161/15168.
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Associate Professor in Dermatology
Epidermis of the skin constitutes the largest organ and the outer most barrier of the body. It is one of the few organs that undergo lifelong self-renewal through a tight balance of cell growth, differentiation, and programmed cell death. Deregulation of this balance is manifested in many diseases, including various immune diseases and cancer. Our lab is focused on 3 interrelated topics: 1. Gene regulation of epithelial cell proliferation and differenti
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