Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice.
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Development of myopia is associated with large-scale changes in ocular tissue gene expression. Although differential expression of coding genes underlying development of myopia has been a subject of intense investigation, the role of non-coding genes such as microRNAs in the development of myopia is largely unknown. In this study, we explored myopia-associated miRNA expression profiles in the retina and sclera of C57Bl/6J mice with experimentally induced myopia using microarray technology. We found a total of 53 differentially expressed miRNAs in the retina and no differences in miRNA expression in the sclera of C57BL/6J mice after 10 days of visual form deprivation, which induced -6.93 ± 2.44 D (p < 0.000001, n = 12) of myopia. We also identified their putative mRNA targets among mRNAs found to be differentially expressed in myopic retina and potential signaling pathways involved in the development of form-deprivation myopia using miRNA-mRNA interaction network analysis. Analysis of myopia-associated signaling pathways revealed that myopic response to visual form deprivation in the retina is regulated by a small number of highly integrated signaling pathways. Our findings highlighted that changes in microRNA expression are involved in the regulation of refractive eye development and predicted how they may be involved in the development of myopia by regulating retinal gene expression.
Disease Models, Animal
Gene Expression Profiling
Gene Regulatory Networks
Mice, Inbred C57BL
Published Version (Please cite this version)10.1371/journal.pone.0162541
Publication InfoTkatchenko, Andrei V; Luo, Xiaoyan; Tkatchenko, Tatiana V; Vaz, Candida; Tanavde, Vivek M; Maurer-Stroh, Sebastian; ... Young, Terri L (2016). Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice. PLoS One, 11(9). pp. e0162541. 10.1371/journal.pone.0162541. Retrieved from https://hdl.handle.net/10161/15319.
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Associate Professor of Ophthalmology
I am interested in understanding the pathologic changes that take place in the trabecular meshwork (the tissue in the eye that is hydrodynamically abnormal in glaucoma). My work is based on the general hypothesis that different types of stress affecting the cells of the trabecular meshwork lead to the accumulation of damage in such cells over many years. This results in loss of cellularity and increase in the number of non-functional cells characterized by a senescent phenotype. <br/
Adjunct Professor in Ophthalmology
Terri L. Young, M.D., M.B.A. is a board-certified clinician-scientist ophthalmologist. She is a Professor of Ophthalmology, Pediatrics, and Medicine at Duke University School of Medicine. She is a Professor of Neuroscience at the Duke- National University of Singapore Graduate Medical School, with adjunct appointments at the Singapore Eye Research Institute and the Saw Swee Hock School of Public Health, National University of Singapore. She is the founding Director of the Duke
Professor in the Department of Mechanical Engineering and Materials Science
My research lies at the intersection of surface and colloid science, polymer materials engineering, and biointerface science, with four central areas of focus: 1. Fabrication, manipulation and characterization of stimulus-responsive biomolecular and bio-inspired polymeric nanostructures on surfaces; 2. Nanotechnology of soft-wet materials and hybrid biological/non-biological microdevices; 3. Receptor-ligand interactions relevant to the diagnostics of infectious diseases; 4. Friction
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