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Loss of cartilage structure, stiffness, and frictional properties in mice lacking PRG4.

dc.contributor.author Coles, JM
dc.contributor.author Zhang, L
dc.contributor.author Blum, JJ
dc.contributor.author Warman, ML
dc.contributor.author Jay, GD
dc.contributor.author Guilak, Farshid
dc.contributor.author Zauscher, Stefan
dc.coverage.spatial United States
dc.date.accessioned 2017-08-18T20:13:33Z
dc.date.available 2017-08-18T20:13:33Z
dc.date.issued 2010-06
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/20191580
dc.identifier.uri http://hdl.handle.net/10161/15321
dc.description.abstract OBJECTIVE: To assess the role of the glycoprotein PRG4 in joint lubrication and chondroprotection by measuring friction, stiffness, surface topography, and subsurface histology of the hip joints of Prg4(-/-) and wild-type (WT) mice. METHODS: Friction and elastic modulus were measured in cartilage from the femoral heads of Prg4(-/-) and WT mice ages 2, 4, 10, and 16 weeks using atomic force microscopy, and the surface microstructure was imaged. Histologic sections of each femoral head were stained and graded. RESULTS: Histologic analysis of the joints of Prg4(-/-) mice showed an enlarged, fragmented surface layer of variable thickness with Safranin O-positive formations sometimes present, a roughened underlying articular cartilage surface, and a progressive loss of pericellular proteoglycans. Friction was significantly higher on cartilage of Prg4(-/-) mice at age 16 weeks, but statistically significant differences in friction were not detected at younger ages. The elastic modulus of the cartilage was similar between cartilage surfaces of Prg4(-/-) and WT mice at young ages, but cartilage of WT mice showed increasing stiffness with age, with significantly higher moduli than cartilage of Prg4(-/-) mice at older ages. CONCLUSION: Deletion of the gene Prg4 results in significant structural and biomechanical changes in the articular cartilage with age, some of which are consistent with osteoarthritic degeneration. These findings suggest that PRG4 plays a significant role in preserving normal joint structure and function.
dc.language eng
dc.relation.ispartof Arthritis Rheum
dc.relation.isversionof 10.1002/art.27436
dc.subject Age Factors
dc.subject Animals
dc.subject Biomechanical Phenomena
dc.subject Cartilage, Articular
dc.subject Elasticity
dc.subject Femur Head
dc.subject Joints
dc.subject Mice
dc.subject Mice, Knockout
dc.subject Microscopy, Atomic Force
dc.subject Proteoglycans
dc.subject Statistics, Nonparametric
dc.subject Surface Properties
dc.subject Synovial Membrane
dc.title Loss of cartilage structure, stiffness, and frictional properties in mice lacking PRG4.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/20191580
pubs.begin-page 1666
pubs.end-page 1674
pubs.issue 6
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Chemistry
pubs.organisational-group Duke
pubs.organisational-group Mechanical Engineering and Materials Science
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 62
dc.identifier.eissn 1529-0131


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