Show simple item record

Female gonadal hormone effects on microglial activation and functional outcomes in a mouse model of moderate traumatic brain injury.

dc.contributor.author Umeano, O
dc.contributor.author Wang, Haichen
dc.contributor.author Dawson, Hana Nenicka
dc.contributor.author Lei, Beilei
dc.contributor.author Umeano, A
dc.contributor.author Kernagis, D
dc.contributor.author James, Michael Lucas
dc.coverage.spatial United States
dc.date.accessioned 2017-09-01T18:04:23Z
dc.date.available 2017-09-01T18:04:23Z
dc.date.issued 2017-05-04
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/28529912
dc.identifier.issn 2220-3141
dc.identifier.uri http://hdl.handle.net/10161/15423
dc.description.abstract AIM: To address the hypothesis that young, gonad-intact female mice have improved long-term recovery associated with decreased neuroinflammation compared to male mice. METHODS: Eight to ten week-old male, female, and ovariectomized (OVX) mice underwent closed cranial impact. Gonad-intact female mice were injured only in estrus state. After injury, between group differences were assessed using complementary immunohistochemical staining for microglial cells at 1 h, mRNA polymerase chain reaction for inflammatory markers at 1 h after injury, Rotarod over days 1-7, and water maze on days 28-31 after injury. RESULTS: Male mice had a greater area of injury (P = 0.0063), F4/80-positive cells (P = 0.032), and up regulation of inflammatory genes compared to female mice. Male and OVX mice had higher mortality after injury when compared to female mice (P = 0.043). No group differences were demonstrated in Rotarod latencies (P = 0.62). OVX mice demonstrated decreased water maze latencies compared to other groups (P = 0.049). CONCLUSION: Differences in mortality, long-term neurological recovery, and markers of neuroinflammation exist between female and male mice after moderate traumatic brain injury (MTBI). Unexpectedly, OVX mice have decreased long term neurological function after MTBI when compared to gonad intact male and female mice. As such, it can be concluded that the presence of female gonadal hormones may influence behavioural outcomes after MTBI, though mechanisms involved are unclear.
dc.language eng
dc.relation.ispartof World J Crit Care Med
dc.relation.isversionof 10.5492/wjccm.v6.i2.107
dc.subject Functional recovery
dc.subject Inflammation
dc.subject Microglia
dc.subject Sex
dc.subject Traumatic brain injury
dc.title Female gonadal hormone effects on microglial activation and functional outcomes in a mouse model of moderate traumatic brain injury.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/28529912
pubs.begin-page 107
pubs.end-page 115
pubs.issue 2
pubs.organisational-group Anesthesiology
pubs.organisational-group Anesthesiology, Neuroanesthesia
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Neurology
pubs.organisational-group Neurology, Neurocritical Care
pubs.organisational-group School of Medicine
pubs.publication-status Published online
pubs.volume 6


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record