(15)N Hyperpolarization of Imidazole-(15)N2 for Magnetic Resonance pH Sensing via SABRE-SHEATH.
Abstract
(15)N nuclear spins of imidazole-(15)N2 were hyperpolarized using NMR signal amplification
by reversible exchange in shield enables alignment transfer to heteronuclei (SABRE-SHEATH).
A (15)N NMR signal enhancement of ∼2000-fold at 9.4 T is reported using parahydrogen
gas (∼50% para-) and ∼0.1 M imidazole-(15)N2 in methanol:aqueous buffer (∼1:1). Proton
binding to a (15)N site of imidazole occurs at physiological pH (pKa ∼ 7.0), and the
binding event changes the (15)N isotropic chemical shift by ∼30 ppm. These properties
are ideal for in vivo pH sensing. Additionally, imidazoles have low toxicity and are
readily incorporated into a wide range of biomolecules. (15)N-Imidazole SABRE-SHEATH
hyperpolarization potentially enables pH sensing on scales ranging from peptide and
protein molecules to living organisms.
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https://hdl.handle.net/10161/15446Published Version (Please cite this version)
10.1021/acssensors.6b00231Publication Info
(2016). (15)N Hyperpolarization of Imidazole-(15)N2 for Magnetic Resonance pH Sensing via
SABRE-SHEATH. ACS Sens, 1(6). pp. 640-644. 10.1021/acssensors.6b00231. Retrieved from https://hdl.handle.net/10161/15446.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Thomas Theis
Assistant Research Professor of Chemistry
Theis' research is at the intersection of Spin Physics and Hyperpolarization Chemistry.
It has applications in the study of biochemical dynamics and molecular imaging. The
Theis lab drives innovation of magnetic resonance tools and techniques to break the
sensitivity limits of NMR and MRI. The innovations enable i) biochemical structure
elucidation with unprecedented limits of detection, and ii) molecular imaging to spy
on mole
Warren S. Warren
James B. Duke Distinguished Professor of Chemistry
Our work focuses on the design and application of what might best be called novel
pulsed techniques, using controlled radiation fields to alter dynamics. The heart
of the work is chemical physics, and most of what we do is ultrafast laser spectroscopy
or nuclear magnetic resonance. It generally involves an intimate mixture of theory
and experiment: recent publications are roughly an equal mix of pencil- and-paper
theory, computer calculations with our workstations, and experiments. Collabo
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