dc.contributor.author |
Pomeroy, Jordan E |
|
dc.contributor.author |
Nguyen, Hung X |
|
dc.contributor.author |
Hoffman, Brenton D |
|
dc.contributor.author |
Bursac, Nenad |
|
dc.coverage.spatial |
Australia |
|
dc.date.accessioned |
2017-09-25T17:55:48Z |
|
dc.date.available |
2017-09-25T17:55:48Z |
|
dc.date.issued |
2017 |
|
dc.identifier |
https://www.ncbi.nlm.nih.gov/pubmed/28912894 |
|
dc.identifier |
thnov07p3539 |
|
dc.identifier.uri |
https://hdl.handle.net/10161/15568 |
|
dc.description.abstract |
Our knowledge of pluripotent stem cell biology has advanced considerably in the past
four decades, but it has yet to deliver on the great promise of regenerative medicine.
The slow progress can be mainly attributed to our incomplete understanding of the
complex biologic processes regulating the dynamic developmental pathways from pluripotency
to fully-differentiated states of functional somatic cells. Much of the difficulty
arises from our lack of specific tools to query, or manipulate, the molecular scale
circuitry on both single-cell and organismal levels. Fortunately, the last two decades
of progress in the field of optogenetics have produced a variety of genetically encoded,
light-mediated tools that enable visualization and control of the spatiotemporal regulation
of cellular function. The merging of optogenetics and pluripotent stem cell biology
could thus be an important step toward realization of the clinical potential of pluripotent
stem cells. In this review, we have surveyed available genetically encoded photoactuators
and photosensors, a rapidly expanding toolbox, with particular attention to those
with utility for studying pluripotent stem cells.
|
|
dc.language |
eng |
|
dc.publisher |
Ivyspring International Publisher |
|
dc.relation.ispartof |
Theranostics |
|
dc.relation.isversionof |
10.7150/thno.20593 |
|
dc.subject |
CRISPR |
|
dc.subject |
optogenetics |
|
dc.subject |
photoactuator |
|
dc.subject |
photosensor |
|
dc.subject |
pluripotent |
|
dc.subject |
stem cell |
|
dc.title |
Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation
of Pluripotent Stem Cells.
|
|
dc.type |
Journal article |
|
duke.contributor.id |
Pomeroy, Jordan E|0655986 |
|
duke.contributor.id |
Hoffman, Brenton D|0575083 |
|
duke.contributor.id |
Bursac, Nenad|0312267 |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/28912894 |
|
pubs.begin-page |
3539 |
|
pubs.end-page |
3558 |
|
pubs.issue |
14 |
|
pubs.organisational-group |
Basic Science Departments |
|
pubs.organisational-group |
Biomedical Engineering |
|
pubs.organisational-group |
Cell Biology |
|
pubs.organisational-group |
Clinical Science Departments |
|
pubs.organisational-group |
Duke |
|
pubs.organisational-group |
Duke Cancer Institute |
|
pubs.organisational-group |
Institutes and Centers |
|
pubs.organisational-group |
Medicine |
|
pubs.organisational-group |
Medicine, Cardiology |
|
pubs.organisational-group |
Pratt School of Engineering |
|
pubs.organisational-group |
School of Medicine |
|
pubs.organisational-group |
Staff |
|
pubs.publication-status |
Published online |
|
pubs.volume |
7 |
|
dc.identifier.eissn |
1838-7640 |
|
duke.contributor.orcid |
Pomeroy, Jordan E|0000-0003-2640-7960 |
|
duke.contributor.orcid |
Bursac, Nenad|0000-0002-5688-6061 |
|