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Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells.

dc.contributor.author Pomeroy, Jordan
dc.contributor.author Nguyen, HX
dc.contributor.author Hoffman, BD
dc.contributor.author Bursac, Nenad
dc.coverage.spatial Australia
dc.date.accessioned 2017-09-25T17:55:48Z
dc.date.available 2017-09-25T17:55:48Z
dc.date.issued 2017
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/28912894
dc.identifier thnov07p3539
dc.identifier.uri https://hdl.handle.net/10161/15568
dc.description.abstract Our knowledge of pluripotent stem cell biology has advanced considerably in the past four decades, but it has yet to deliver on the great promise of regenerative medicine. The slow progress can be mainly attributed to our incomplete understanding of the complex biologic processes regulating the dynamic developmental pathways from pluripotency to fully-differentiated states of functional somatic cells. Much of the difficulty arises from our lack of specific tools to query, or manipulate, the molecular scale circuitry on both single-cell and organismal levels. Fortunately, the last two decades of progress in the field of optogenetics have produced a variety of genetically encoded, light-mediated tools that enable visualization and control of the spatiotemporal regulation of cellular function. The merging of optogenetics and pluripotent stem cell biology could thus be an important step toward realization of the clinical potential of pluripotent stem cells. In this review, we have surveyed available genetically encoded photoactuators and photosensors, a rapidly expanding toolbox, with particular attention to those with utility for studying pluripotent stem cells.
dc.language eng
dc.relation.ispartof Theranostics
dc.relation.isversionof 10.7150/thno.20593
dc.subject CRISPR
dc.subject optogenetics
dc.subject photoactuator
dc.subject photosensor
dc.subject pluripotent
dc.subject stem cell
dc.title Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/28912894
pubs.begin-page 3539
pubs.end-page 3558
pubs.issue 14
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Cell Biology
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group Staff
pubs.publication-status Published online
pubs.volume 7
dc.identifier.eissn 1838-7640


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