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RanBP2 modulates Cox11 and hexokinase I activities and haploinsufficiency of RanBP2 causes deficits in glucose metabolism.

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Date
2006-10
Authors
Aslanukov, Azamat
Bhowmick, Reshma
Guruju, Mallikarjuna
Oswald, John
Raz, Dorit
Bush, Ronald A
Sieving, Paul A
Lu, Xinrong
Bock, Cheryl B
Ferreira, Paulo A
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Abstract
The Ran-binding protein 2 (RanBP2) is a large multimodular and pleiotropic protein. Several molecular partners with distinct functions interacting specifically with selective modules of RanBP2 have been identified. Yet, the significance of these interactions with RanBP2 and the genetic and physiological role(s) of RanBP2 in a whole-animal model remain elusive. Here, we report the identification of two novel partners of RanBP2 and a novel physiological role of RanBP2 in a mouse model. RanBP2 associates in vitro and in vivo and colocalizes with the mitochondrial metallochaperone, Cox11, and the pacemaker of glycolysis, hexokinase type I (HKI) via its leucine-rich domain. The leucine-rich domain of RanBP2 also exhibits strong chaperone activity toward intermediate and mature folding species of Cox11 supporting a chaperone role of RanBP2 in the cytosol during Cox11 biogenesis. Cox11 partially colocalizes with HKI, thus supporting additional and distinct roles in cell function. Cox11 is a strong inhibitor of HKI, and RanBP2 suppresses the inhibitory activity of Cox11 over HKI. To probe the physiological role of RanBP2 and its role in HKI function, a mouse model harboring a genetically disrupted RanBP2 locus was generated. RanBP2(-/-) are embryonically lethal, and haploinsufficiency of RanBP2 in an inbred strain causes a pronounced decrease of HKI and ATP levels selectively in the central nervous system. Inbred RanBP2(+/-) mice also exhibit deficits in growth rates and glucose catabolism without impairment of glucose uptake and gluconeogenesis. These phenotypes are accompanied by a decrease in the electrophysiological responses of photosensory and postreceptoral neurons. Hence, RanBP2 and its partners emerge as critical modulators of neuronal HKI, glucose catabolism, energy homeostasis, and targets for metabolic, aging disorders and allied neuropathies.
Type
Journal article
Subject
Amino Acid Sequence
Animals
Cells, Cultured
Electroretinography
Glucose
HSP70 Heat-Shock Proteins
Haploidy
Hexokinase
Membrane Proteins
Mice
Mice, Mutant Strains
Mitochondrial Proteins
Models, Biological
Molecular Chaperones
Molecular Sequence Data
Mutagenesis, Insertional
Nuclear Pore Complex Proteins
Phenotype
Photoreceptor Cells
Protein Binding
Protein Structure, Tertiary
Protein Transport
Structure-Activity Relationship
Permalink
https://hdl.handle.net/10161/15575
Published Version (Please cite this version)
10.1371/journal.pgen.0020177
Publication Info
Aslanukov, Azamat; Bhowmick, Reshma; Guruju, Mallikarjuna; Oswald, John; Raz, Dorit; Bush, Ronald A; ... Ferreira, Paulo A (2006). RanBP2 modulates Cox11 and hexokinase I activities and haploinsufficiency of RanBP2 causes deficits in glucose metabolism. PLoS Genet, 2(10). pp. e177. 10.1371/journal.pgen.0020177. Retrieved from https://hdl.handle.net/10161/15575.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Ferreira

Paulo Alexandre Ferreira

Associate Professor in Ophthalmology
The long-term goal of our research program is twofold. The first is to understand the interplay between intracellular signaling, intracellular trafficking and proteostasis in health and disease; the second is to uncover molecular players and mechanisms partaking in such processes that are amenable to therapeutic intervention in a variety of disease states. Presently, our research efforts are centered on dissecting the roles of two disease-associated protein interactomes assembled by the Ran
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