CXCL10 is Upregulated in Synovium and Cartilage following Articular Fracture.
Abstract
The objective of this study was to investigate the expression of the chemokine CXCL10
and its role in joint tissues following articular fracture. We hypothesized that CXCL10
is upregulated following articular fracture and contributes to cartilage degradation
associated with post-traumatic arthritis (PTA). To evaluate CXCL10 expression following
articular fracture, gene expression was quantified in synovial tissue from knee joints
of C57BL/6 mice that develop PTA following articular fracture, and MRL/MpJ mice that
are protected from PTA. CXCL10 protein expression was assessed in human cartilage
in normal, osteoarthritic (OA), and post-traumatic tissue using immunohistochemistry.
The effects of exogenous CXCL10, alone and in combination with IL-1, on porcine cartilage
explants were assessed by quantifying the release of catabolic mediators. Synovial
tissue gene expression of CXCL10 was upregulated by joint trauma, peaking one day
in C57BL/6 mice (25-fold) vs. three days post-fracture in MRL/MpJ mice (15-fold).
CXCL10 protein in articular cartilage was most highly expressed following trauma compared
with normal and OA tissue. In a dose dependent manner, exogenous CXCL10 significantly
reduced total matrix metalloproteinase (MMP) and aggrecanase activity of culture media
from cartilage explants. CXCL10 also trended toward a reduction in IL-1α-stimulated
total MMP activity (p=0.09) and S-GAG (p=0.09), but not NO release. In conclusion,
CXCL10 was upregulated in synovium and chondrocytes following trauma. However, exogenous
CXCL10 did not induce a catabolic response in cartilage. CXCL10 may play a role in
modulating the chondrocyte response to inflammatory stimuli associated with joint
injury and the progression of PTA. This article is protected by copyright. All rights
reserved.
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https://hdl.handle.net/10161/15588Published Version (Please cite this version)
10.1002/jor.23735Publication Info
Furman, Bridgette D; Kent, Collin L; Huebner, Janet L; Kraus, Virginia B; McNulty,
Amy L; Guilak, Farshid; & Olson, Steven A (2017). CXCL10 is Upregulated in Synovium and Cartilage following Articular Fracture. J Orthop Res. 10.1002/jor.23735. Retrieved from https://hdl.handle.net/10161/15588.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Farshid Guilak
Lazlo Ormandy Professor of Orthopaedic Surgery
This author no longer has a Scholars@Duke profile, so the information shown here reflects
their Duke status at the time this item was deposited.
Virginia Byers Kraus
Professor of Medicine
My special area of expertise is as a clinician scientist investigating osteoarthritis.
Osteoarthritis is the most common form of joint disease in man and its incidence increases
with age. It is a problem of increasing concern to the medical community due to the
increasing longevity of the population. Trained as a molecular biologist and a Rheumatologist,
I endeavor to study this disease from bedside to bench. The work in this laboratory
focuses on osteoarthritis and deals w
Amy Lynn McNulty
Associate Professor in Orthopaedic Surgery
The McNulty Lab is working to develop strategies to prevent osteoarthritis and to
promote tissue repair and regeneration following joint injury. In order to accomplish
this, we are working in three main areas. 1) We are working to understand the pathways
that are activated by normal and injurious mechanical loading of cartilage and meniscus
and how these mechanotransduction pathways are altered during aging, injury, and tissue
degeneration. A greater understanding of alterations in mech
Steven Arthur Olson
Professor of Orthopaedic Surgery
As an Orthopaedic Surgeon my primary focus of research is joint preservation. My
primary clinical interests are Orthopaedic Trauma and Hip Reconstruction. In Orthopaedic
Trauma my research interests are 1) Basic science investigations of articular fractures
with two current animal models in use. 2) Clinical research includes evaluation of
techniques to reduce and stabilize articular fractures, as well as management of open
fractures. In the area of Hip Reconstruction
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