CXCL10 is Upregulated in Synovium and Cartilage following Articular Fracture.
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The objective of this study was to investigate the expression of the chemokine CXCL10 and its role in joint tissues following articular fracture. We hypothesized that CXCL10 is upregulated following articular fracture and contributes to cartilage degradation associated with post-traumatic arthritis (PTA). To evaluate CXCL10 expression following articular fracture, gene expression was quantified in synovial tissue from knee joints of C57BL/6 mice that develop PTA following articular fracture, and MRL/MpJ mice that are protected from PTA. CXCL10 protein expression was assessed in human cartilage in normal, osteoarthritic (OA), and post-traumatic tissue using immunohistochemistry. The effects of exogenous CXCL10, alone and in combination with IL-1, on porcine cartilage explants were assessed by quantifying the release of catabolic mediators. Synovial tissue gene expression of CXCL10 was upregulated by joint trauma, peaking one day in C57BL/6 mice (25-fold) vs. three days post-fracture in MRL/MpJ mice (15-fold). CXCL10 protein in articular cartilage was most highly expressed following trauma compared with normal and OA tissue. In a dose dependent manner, exogenous CXCL10 significantly reduced total matrix metalloproteinase (MMP) and aggrecanase activity of culture media from cartilage explants. CXCL10 also trended toward a reduction in IL-1α-stimulated total MMP activity (p=0.09) and S-GAG (p=0.09), but not NO release. In conclusion, CXCL10 was upregulated in synovium and chondrocytes following trauma. However, exogenous CXCL10 did not induce a catabolic response in cartilage. CXCL10 may play a role in modulating the chondrocyte response to inflammatory stimuli associated with joint injury and the progression of PTA. This article is protected by copyright. All rights reserved.
Published Version (Please cite this version)10.1002/jor.23735
Publication InfoFurman, BD; Guilak, Farshid; Huebner, JL; Kent, CL; Kraus, Virginia Byers; McNulty, Amy; & Olson, Steven Arthur (2017). CXCL10 is Upregulated in Synovium and Cartilage following Articular Fracture. J Orthop Res. 10.1002/jor.23735. Retrieved from http://hdl.handle.net/10161/15588.
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Lazlo Ormandy Professor of Orthopaedic Surgery
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.
Professor of Medicine
My special area of expertise is as a clinician scientist investigating osteoarthritis. Osteoarthritis is the most common form of joint disease in man and its incidence increases with age. It is a problem of increasing concern to the medical community due to the increasing longevity of the population. Trained as a molecular biologist and a Rheumatologist, I endeavor to study this disease from bedside to bench. The work in this laboratory focuses on osteoarthritis and deals w
Associate Professor in Orthopaedic Surgery
The long term goals of the McNulty lab are to develop strategies to prevent osteoarthritis and to promote tissue repair and regeneration following joint injury. In order to achieve these goals, we need to understand the mechanisms necessary for tissue repair and regeneration and how they are altered with aging and joint injury. Specifically, we are working to enhance the integrative repair of meniscus to restore meniscal function and decrease the risk of osteoarthritis development. &
Professor of Orthopaedic Surgery
As an Orthopaedic Surgeon my primary focus of research is joint preservation. My primary clinical interests are Orthopaedic Trauma and Hip Reconstruction. In Orthopaedic Trauma my research interests are 1) Basic science investigations of articular fractures with two current animal models in use. 2) Clinical research includes evaluation of techniques to reduce and stabilize articular fractures, as well as management of open fractures. In the area of Hip Reconstruction
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