Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.
Abstract
Protein microarray technology has been successfully used for identifying substrates
of purified activated kinases. We used protein microarrays to globally interrogate
the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo
glioma models and validated results in clinical pathological specimens. Whole cell
lysates extracted from tumor samples can be applied to human kinome chip microarrays
to profile the global kinase phosphorylation patterns in a high-throughput manner
and identify novel substrates inherent to the tumor cell and the interactions with
tumor microenvironment. Our findings identify a novel microarray-based method for
assessing intracellular signaling events applicable to human oncogenesis and other
pathophysiologic states.
Type
Journal articlePermalink
https://hdl.handle.net/10161/15820Published Version (Please cite this version)
10.1016/j.tranon.2016.02.001Publication Info
Goodwin, C Rory; Woodard, Crystal L; Zhou, Xin; Pan, Jianbo; Olivi, Alessandro; Xia,
Shuli; ... Laterra, John (2016). Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems. Transl Oncol, 9(2). pp. 124-129. 10.1016/j.tranon.2016.02.001. Retrieved from https://hdl.handle.net/10161/15820.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Courtney Rory Goodwin
Assistant Professor of Neurosurgery

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