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Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.

dc.contributor.author Chien, JS
dc.contributor.author Eldik, Hysem
dc.contributor.author Ibrahim, Mohamed
dc.contributor.author Klitzman, Bruce
dc.contributor.author Martinez, J
dc.contributor.author Mohammed, Mahmoud
dc.contributor.author Nichols, Scott P
dc.contributor.author Wisniewski, NA
dc.coverage.spatial USA
dc.date.accessioned 2017-08-25T22:02:24Z
dc.date.accessioned 2018-01-22T17:15:44Z
dc.date.available 2018-01-22T17:15:44Z
dc.date.issued 2017-08-15
dc.identifier.uri https://hdl.handle.net/10161/16012
dc.description.abstract Novel injectable biosensors were used to measure interstitial oxygenation before, during, and after transient ischemia. It is well known that reactive hyperemia occurs following a period of ischemia. However, increased blood flow does not necessarily mean increased oxygen tension in the tissue. Therefore, the purpose of this study was to test the hypothesis that tissue reactive hyperoxia occurs following release of hind-limb tourniquet occlusions. Rats were injected with bilateral hindlimb biosensors and were simultaneously subjected to a unilateral femoral vessel ligation. After approximately one and three months, the rats underwent a series of oxygenation challenges, including transient hind-limb tourniquet occlusion. Along with the biosensors, near infrared spectroscopy was used to measure percent oxyhemoglobin in capillaries and laser Doppler flowmetry was used to measure blood flow. Post-occlusion reactive hyperemia was observed. It was accompanied by tissue reactive hyperoxia, affirming that the post-occlusion oxygen supply must have exceeded the expected increased oxygen consumption. The measurement of the physiologic phenomenon of reactive hyperoxia could prove clinically beneficial for both diagnosis and optimizing therapy.
dc.publisher Springer Nature
dc.relation.ispartof Nature Scientific Reports
dc.relation.isversionof 10.1038/s41598-017-08490-0.
dc.relation.replaces http://hdl.handle.net/10161/15361
dc.relation.replaces 10161/15361
dc.subject oxygen sensor
dc.subject implantable
dc.title Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.
dc.type Journal article
pubs.author-url doi:%2010.1038/s41598-017-08490-0.
pubs.begin-page 1
pubs.end-page 10
pubs.issue 1
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Cell Biology
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group Surgery
pubs.organisational-group Surgery, Plastic, Maxillofacial, and Oral Surgery
pubs.publication-status Published online
pubs.volume 7


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