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FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings.

dc.contributor.author Erickson, Harold Paul
dc.contributor.author Housman, Max
dc.contributor.author Milam, Sara L
dc.contributor.author Moore, Desmond A
dc.contributor.author Osawa, Masaki
dc.date.accessioned 2018-04-01T14:45:33Z
dc.date.available 2018-04-01T14:45:33Z
dc.date.issued 2016-07
dc.identifier.issn 0006-2960
dc.identifier.issn 1520-4995
dc.identifier.uri https://hdl.handle.net/10161/16450
dc.description.abstract FtsZ protofilaments (pfs) form the bacterial cytokinetic Z ring. Previous work suggested that a conformational change from straight to curved pfs generated the constriction force. In the simplest model, the C-terminal membrane tether is on the outside of the curved pf, facing the membrane. Tubulin, a homologue of FtsZ, also forms pfs with a curved conformation. However, it is well-established that tubulin rings have the C terminus on the inside of the ring. Could FtsZ and tubulin rings have the opposite curvature? In this study, we explored the FtsZ curvature direction by fusing large protein tags to the FtsZ termini. Thin section electron microscopy showed that the C-terminal tag was on the outside, consistent with the bending pf model. This has interesting implications for the evolution of tubulin. Tubulin likely began with the curvature of FtsZ, but evolution managed to reverse direction to produce outward-curving rings, which are useful for pulling chromosomes.
dc.language eng
dc.relation.ispartof Biochemistry
dc.relation.isversionof 10.1021/acs.biochem.6b00479
dc.subject Multiprotein Complexes
dc.subject Tubulin
dc.subject Peptidoglycan
dc.subject Bacterial Proteins
dc.subject Escherichia coli Proteins
dc.subject Cytoskeletal Proteins
dc.subject Microscopy, Electron
dc.subject Protein Conformation
dc.subject Models, Molecular
dc.subject Protein Multimerization
dc.title FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings.
dc.type Journal article
dc.date.updated 2018-04-01T14:45:31Z
pubs.issue 29
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Biochemistry
pubs.organisational-group Basic Science Departments
pubs.organisational-group Cell Biology
pubs.publication-status Published
pubs.volume 55


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