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FtsZ filament capping by MciZ, a developmental regulator of bacterial division.
Abstract
Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins.
FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that
polymerizes into rings localized to cell-division sites, and the constriction of these
rings drives cytokinesis. Here we investigate the mechanism by which the Bacillus
subtilis cell-division inhibitor, MciZ (mother cell inhibitor of FtsZ), blocks assembly
of FtsZ. The X-ray crystal structure reveals that MciZ binds to the C-terminal polymerization
interface of FtsZ, the equivalent of the minus end of tubulin. Using in vivo and in
vitro assays and microscopy, we show that MciZ, at substoichiometric levels to FtsZ,
causes shortening of protofilaments and blocks the assembly of higher-order FtsZ structures.
The findings demonstrate an unanticipated capping-based regulatory mechanism for FtsZ.
Type
Journal articleSubject
Bacillus subtilisBacterial Proteins
Cell Cycle Proteins
Cytoskeletal Proteins
Crystallography, X-Ray
Protein Structure, Quaternary
Protein Structure, Tertiary
Permalink
https://hdl.handle.net/10161/16451Published Version (Please cite this version)
10.1073/pnas.1414242112Publication Info
Bisson-Filho, Alexandre W; Discola, Karen F; Castellen, Patrícia; Blasios, Valdir;
Martins, Alexandre; Sforça, Maurício L; ... Gueiros-Filho, Frederico J (2015). FtsZ filament capping by MciZ, a developmental regulator of bacterial division. Proceedings of the National Academy of Sciences of the United States of America, 112(17). 10.1073/pnas.1414242112. Retrieved from https://hdl.handle.net/10161/16451.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Harold Paul Erickson
James B. Duke Distinguished Professor Emeritus
Recent research has been on cytoskeleton (eukaryotes and bacteria); a skirmish to
debunk the irisin story; a reinterpretation of proposed multivalent binders of the
coronavirus spike protein. I have also published an ebook on "Principles of Protein-Protein
Association" suitable for a course module or individual learning.

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