Show simple item record

Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew.

dc.contributor.author Johnson, Elizabeth
dc.contributor.author Field, Greg
dc.contributor.author Westbrook, Teleza
dc.contributor.author Shayesteh, Rod
dc.contributor.author Chen, Emily L
dc.contributor.author Schumacher, Joseph W
dc.contributor.author Fitzpatrick, David
dc.date.accessioned 2018-05-01T13:34:39Z
dc.date.available 2018-05-01T13:34:39Z
dc.date.issued 2017-12-14
dc.identifier.issn 0021-9967
dc.identifier.issn 1096-9861
dc.identifier.uri https://hdl.handle.net/10161/16625
dc.description.abstract Intrinsically photosensitive retinal ganglion cells (ipRGCs) mediate the pupillary light reflex, circadian entrainment, and may contribute to luminance and color perception. The diversity of ipRGCs varies from rodents to primates, suggesting differences in their contributions to retinal output. To further understand the variability in their organization and diversity across species, we used immunohistochemical methods to examine ipRGCs in tree shrew (Tupaia belangeri). Tree shrews share membership in the same clade, or evolutionary branch, as rodents and primates. They are highly visual, diurnal animals with a cone-dominated retina and a geniculo-cortical organization resembling that of primates. We identified cells with morphological similarities to M1 and M2 cells described previously in rodents and primates. M1-like cells typically had somas in the ganglion cell layer, with 23% displaced to the inner nuclear layer (INL). However, unlike M1 cells, they had bistratified dendritic fields ramifying in S1 and S5 that collectively tiled space. M2-like cells had dendritic fields restricted to S5 that were smaller and more densely branching. A novel third type of melanopsin immunopositive cell was identified. These cells had somata exclusively in the INL and monostratified dendritic fields restricted to S1 that tiled space. Surprisingly, these cells immunolabeled for tyrosine hydroxylase, a key component in dopamine synthesis. These cells immunolabeled for an RGC marker, not amacrine cell markers, suggesting that they are dopaminergic ipRGCs. We found no evidence for M4 or M5 ipRGCs, described previously in rodents. These results identify some organizational features of the ipRGC system that are canonical versus species-specific.
dc.language eng
dc.relation.ispartof The Journal of comparative neurology
dc.relation.isversionof 10.1002/cne.24377
dc.subject circadian
dc.subject dopamine
dc.subject melanopsin
dc.subject myopia
dc.subject nile rat
dc.subject opn4
dc.subject tyrosine hydroxylase
dc.title Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew.
dc.type Journal article
dc.date.updated 2018-05-01T13:34:36Z
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Neurobiology
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group University Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.publication-status Published


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record