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Cell type-specific changes in retinal ganglion cell function induced by rod death and cone reorganization in rats.

dc.contributor.author Field, Greg
dc.contributor.author Yu, Wan-Qing
dc.contributor.author Grzywacz, Norberto M
dc.contributor.author Lee, Eun-Jin
dc.date.accessioned 2018-05-01T13:35:11Z
dc.date.available 2018-05-01T13:35:11Z
dc.date.issued 2017-07
dc.identifier.issn 0022-3077
dc.identifier.issn 1522-1598
dc.identifier.uri https://hdl.handle.net/10161/16626
dc.description.abstract We have determined the impact of rod death and cone reorganization on the spatiotemporal receptive fields (RFs) and spontaneous activity of distinct retinal ganglion cell (RGC) types. We compared RGC function between healthy and retinitis pigmentosa (RP) model rats (S334ter-3) at a time when nearly all rods were lost but cones remained. This allowed us to determine the impact of rod death on cone-mediated visual signaling, a relevant time point because the diagnosis of RP frequently occurs when patients are nightblind but daytime vision persists. Following rod death, functionally distinct RGC types persisted; this indicates that parallel processing of visual input remained largely intact. However, some properties of cone-mediated responses were altered ubiquitously across RGC types, such as prolonged temporal integration and reduced spatial RF area. Other properties changed in a cell type-specific manner, such as temporal RF shape (dynamics), spontaneous activity, and direction selectivity. These observations identify the extent of functional remodeling in the retina following rod death but before cone loss. They also indicate new potential challenges to restoring normal vision by replacing lost rod photoreceptors.NEW & NOTEWORTHY This study provides novel and therapeutically relevant insights to retinal function following rod death but before cone death. To determine changes in retinal output, we used a large-scale multielectrode array to simultaneously record from hundreds of retinal ganglion cells (RGCs). These recordings of large-scale neural activity revealed that following the death of all rods, functionally distinct RGCs remain. However, the receptive field properties and spontaneous activity of these RGCs are altered in a cell type-specific manner.
dc.language eng
dc.relation.ispartof Journal of neurophysiology
dc.relation.isversionof 10.1152/jn.00826.2016
dc.subject Retinal Ganglion Cells
dc.subject Animals
dc.subject Rats, Long-Evans
dc.subject Rats, Sprague-Dawley
dc.subject Retinitis Pigmentosa
dc.subject Disease Models, Animal
dc.subject Tissue Culture Techniques
dc.subject Cell Death
dc.subject Action Potentials
dc.subject Neuronal Plasticity
dc.subject Female
dc.subject Male
dc.subject Vision, Ocular
dc.subject Retinal Rod Photoreceptor Cells
dc.subject Retinal Cone Photoreceptor Cells
dc.title Cell type-specific changes in retinal ganglion cell function induced by rod death and cone reorganization in rats.
dc.type Journal article
dc.date.updated 2018-05-01T13:35:10Z
pubs.issue 1
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Neurobiology
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group University Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.publication-status Published
pubs.volume 118


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