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Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia.
Abstract
Novel injectable biosensors were used to measure interstitial oxygenation before,
during, and after transient ischemia. It is well known that reactive hyperemia occurs
following a period of ischemia. However, increased blood flow does not necessarily
mean increased oxygen tension in the tissue. Therefore, the purpose of this study
was to test the hypothesis that tissue reactive hyperoxia occurs following release
of hind-limb tourniquet occlusions. Rats were injected with bilateral hind-limb biosensors
and were simultaneously subjected to a unilateral femoral vessel ligation. After approximately
one and three months, the rats underwent a series of oxygenation challenges, including
transient hind-limb tourniquet occlusion. Along with the biosensors, near infrared
spectroscopy was used to measure percent oxyhemoglobin in capillaries and laser Doppler
flowmetry was used to measure blood flow. Post-occlusion reactive hyperemia was observed.
It was accompanied by tissue reactive hyperoxia, affirming that the post-occlusion
oxygen supply must have exceeded the expected increased oxygen consumption. The measurement
of the physiologic phenomenon of reactive hyperoxia could prove clinically beneficial
for both diagnosis and optimizing therapy.
Type
Journal articlePermalink
https://hdl.handle.net/10161/17080Published Version (Please cite this version)
10.1038/s41598-017-08490-0Publication Info
Chien, Jennifer S; Mohammed, Mahmoud; Eldik, Hysem; Ibrahim, Mohamed M; Martinez,
Jeremy; Nichols, Scott P; ... Klitzman, Bruce (2017). Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive
Hyperoxia. Scientific reports, 7(1). 10.1038/s41598-017-08490-0. Retrieved from https://hdl.handle.net/10161/17080.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Bruce Klitzman
Associate Professor Emeritus in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors,
and long term improvement of medical device implantation. My basic research interests
are in the area of physiological mechanisms of optimizing substrate transport to tissue.
This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular,
cellular, ultrastructural, and molecular level. The current projects include:
1) control of blood flow and flow distribu

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