The potential repertoire of the innate immune system in the bladder: expression of pattern recognition receptors in the rat bladder and a rat urothelial cell line (MYP3 cells).
Abstract
The urothelium is a frontline sensor of the lower urinary tract, sampling the bladder
lumen and stimulating an immune response to infectious and noxious agents. Pattern
recognition receptors (PRRs) recognize such agents and coordinate the innate response,
often by forming inflammasomes that activate caspase-1 and the release of interleukin-1.
We have shown the presence of one PRR (NLRP3) in the urothelia and its central role
in the inflammatory response to cyclophosphamide. The purpose of this study was to
(1) assess the likely range of the PPR response by assessing the repertoire present
in the rat bladder and (2) determine the utility of the MYP3 rat urothelia cell line
for in vitro studies by assessing its PPR repertoire and functional responsiveness.Immunohistochemistry
was performed for seven PPRs (NLRP1, NLRP3, NLRP6, NLRP7, NLRP12, NLRC4 and AIM2)
on bladder sections and MYP3 cells. For functionality, MYP3 cells were challenged
with the quintessential NLRP3 activator ATP and assessed for caspase-1 activation.All
PPRs examined were expressed in the bladder and localized to the urothelial layer
with several also in the detrusor (none in the interstitia). MYP3 cells also expressed
all PRRs with a variable intracellular location. ATP-stimulated caspase-1 activity
in MYP3 cells in a dose-dependent manner was reduced by knockdown of NLRP3 expression.The
results suggest that the bladder possesses the capacity to initiate an innate immune
response to a wide array of uropathological agents and the MYP3 cells will provide
an excellent investigational tool for this field.
Type
Journal articleSubject
UrotheliumCell Line
Animals
Rats
Rats, Sprague-Dawley
Caspase 1
Carrier Proteins
DNA-Binding Proteins
Receptors, Cell Surface
Receptors, Angiotensin
Receptors, Vasopressin
Nerve Tissue Proteins
RNA, Small Interfering
Adenosine Triphosphate
Female
Receptors, Pattern Recognition
Urinary Bladder
Immunity, Innate
Gene Knockdown Techniques
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https://hdl.handle.net/10161/17177Published Version (Please cite this version)
10.1007/s11255-015-1126-6Publication Info
Hughes, Francis M; Turner, David P; & Todd Purves, J (2015). The potential repertoire of the innate immune system in the bladder: expression of
pattern recognition receptors in the rat bladder and a rat urothelial cell line (MYP3
cells). International urology and nephrology, 47(12). pp. 1953-1964. 10.1007/s11255-015-1126-6. Retrieved from https://hdl.handle.net/10161/17177.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Monty Hughes Jr.
Assistant Professor in Surgery
Dr. Hughes received his Ph.D. from the Medical University of South Carolina and was
a post doc at both the University of North Carolina at Chapel Hill and NIH. He then
joined the faculty of the University of North Carolina at Charlotte where he rose
to the rank of Associate Professor (with tenure). Following a brief stint as the director
of the biology division of a start-up pharmaceutical company, he joined forces with
Dr. Purves at the Medical University of South Carolina to begin this l

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