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Role of NHERF and scaffolding proteins in proximal tubule transport.

dc.contributor.author Biswas, Rajatsubhra
dc.contributor.author Cunningham, Rochelle
dc.contributor.author Shenolikar, Shirish
dc.contributor.author Steplock, Deborah
dc.contributor.author Weinman, Edward J
dc.date.accessioned 2018-07-16T16:53:21Z
dc.date.available 2018-07-16T16:53:21Z
dc.date.issued 2010-08
dc.identifier.issn 0300-5623
dc.identifier.issn 1434-0879
dc.identifier.uri https://hdl.handle.net/10161/17229
dc.description.abstract Eukaryotic cells coordinate specific responses to hormones and growth factors by spatial and temporal organization of "signaling components." Through the formation of multiprotein complexes, cells are able to generate "signaling components" that transduce hormone signals through proteins, such as PSD-95/Dlg/ZO-1(PDZ)-containing proteins that associate by stable and dynamic interactions. The PDZ homology domain is a common protein interaction domain in eukaryotes and with greater than 500 PDZ domains identified, it is the most abundant protein interaction domain in eukaryotic cells. The NHERF (sodium hydrogen exchanger regulatory factor) proteins are PDZ domain-containing proteins that play an important role in maintaining and regulating cell function. NHERF-1 was initially identified as a brush border membrane-associated phosphoprotein essential for the cAMP/PKA-induced inhibition of the sodium hydrogen exchanger isoform 3 (NHE3). Mouse, rabbit and human renal proximal tubules also express NHERF-2 (E3KARP), a structurally related protein, which in model cell systems also binds NHE3 and mediates its inhibition by cAMP. PDZK1 (NHERF-3) and IKEPP (NHERF-4) were later identified and found to have similar homology domains, leading to their recent reclassification. Although studies have revealed similar binding partners and overlapping functions for the NHERF proteins, it is clear that there is a significant amount of specificity between them. This review focuses primarily on NHERF-1, as the prototypical PDZ protein and will give a brief summary of its role in phosphate transport and the development of some forms of nephrolithiasis.
dc.language eng
dc.publisher SPRINGER
dc.relation.ispartof Urological research
dc.relation.isversionof 10.1007/s00240-010-0294-1
dc.subject Kidney Tubules, Proximal
dc.subject Animals
dc.subject Rabbits
dc.subject Humans
dc.subject Mice
dc.subject Phosphates
dc.subject Sodium-Hydrogen Antiporter
dc.subject Phosphoproteins
dc.subject Microscopy, Confocal
dc.subject Signal Transduction
dc.subject Protein Binding
dc.subject PDZ Domains
dc.title Role of NHERF and scaffolding proteins in proximal tubule transport.
dc.type Journal article
dc.date.updated 2018-07-16T16:53:20Z
pubs.begin-page 257
pubs.end-page 262
pubs.issue 4
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Psychiatry & Behavioral Sciences, Translational Neuroscience
pubs.organisational-group Psychiatry & Behavioral Sciences
pubs.organisational-group Clinical Science Departments
pubs.publication-status Published
pubs.volume 38
duke.contributor.orcid Shenolikar, Shirish|0000-0003-0540-6328


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