Skewing of the population balance of lymphoid and myeloid cells by secreted and intracellular osteopontin.
Abstract
The balance of myeloid populations and lymphoid populations must be well controlled.
Here we found that osteopontin (OPN) skewed this balance during pathogenic conditions
such as infection and autoimmunity. Notably, two isoforms of OPN exerted distinct
effects in shifting this balance through cell-type-specific regulation of apoptosis.
Intracellular OPN (iOPN) diminished the population size of myeloid progenitor cells
and myeloid cells, and secreted OPN (sOPN) increase the population size of lymphoid
cells. The total effect of OPN on skewing the leukocyte population balance was observed
as host sensitivity to early systemic infection with Candida albicans and T cell-mediated
colitis. Our study suggests previously unknown detrimental roles for two OPN isoforms
in causing the imbalance of leukocyte populations.
Type
Journal articleSubject
LymphocytesT-Lymphocytes
Myeloid Cells
Animals
Mice, Knockout
Mice
Candida albicans
Infection
Candidiasis
Colitis
Autoimmune Diseases
Protein Isoforms
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Reverse Transcriptase Polymerase Chain Reaction
Apoptosis
Lymphopoiesis
Myelopoiesis
Cell Proliferation
Osteopontin
Real-Time Polymerase Chain Reaction
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https://hdl.handle.net/10161/17648Published Version (Please cite this version)
10.1038/ni.3791Publication Info
Kanayama, Masashi; Xu, Shengjie; Danzaki, Keiko; Gibson, Jason R; Inoue, Makoto; Gregory,
Simon G; & Shinohara, Mari L (2017). Skewing of the population balance of lymphoid and myeloid cells by secreted and intracellular
osteopontin. Nature immunology, 18(9). pp. 973-984. 10.1038/ni.3791. Retrieved from https://hdl.handle.net/10161/17648.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Simon Gray Gregory
Professor in Neurosurgery
Dr. Gregory is a tenured Professor and Director of the Brain Tumor Omics Program (BTOP)
in the Duke Department of Neurosurgery, the Vice Chair of Research in the Department
of Neurology, and Director of the Molecular Genomics Core at the Duke Molecular Physiology
Institute.
As a neurogenomicist, Dr. Gregory applies the experience gained from leading the sequencing
of chromosome 1 for the Human Genome Project to elucidating the mechanisms underlying
multi-factorial
Mari L. Shinohara
Professor of Integrative Immunobiology
Shinohara Lab WebsiteImmune responses against pathogens are essential for host protection,
but excessive and uncontrolled immune reactions can lead to autoimmunity. How does
our immune system keep the balance fine-tuned? This is a central question being asked
in my laboratory.
The immune system needs to detect pathogens quickly and effectively. This is performed
by the innate immune system, which includes cells such as mac
Shengjie Xu
Research Assistant, Ph D Student
Program Start Year: 2013Mari Shinohara Laboratory"Novel Heterogeneous Alveolar Macrophage
Subpopulations during Early Fungal Infection"
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