Whole Exome Sequencing Identifies Frequent Somatic Mutations in Cell-Cell Adhesion Genes in Chinese Patients with Lung Squamous Cell Carcinoma.
Abstract
Lung squamous cell carcinoma (SQCC) accounts for about 30% of all lung cancer cases.
Understanding of mutational landscape for this subtype of lung cancer in Chinese patients
is currently limited. We performed whole exome sequencing in samples from 100 patients
with lung SQCCs to search for somatic mutations and the subsequent target capture
sequencing in another 98 samples for validation. We identified 20 significantly mutated
genes, including TP53, CDH10, NFE2L2 and PTEN. Pathways with frequently mutated genes
included those of cell-cell adhesion/Wnt/Hippo in 76%, oxidative stress response in
21%, and phosphatidylinositol-3-OH kinase in 36% of the tested tumor samples. Mutations
of Chromatin regulatory factor genes were identified at a lower frequency. In functional
assays, we observed that knockdown of CDH10 promoted cell proliferation, soft-agar
colony formation, cell migration and cell invasion, and overexpression of CDH10 inhibited
cell proliferation. This mutational landscape of lung SQCC in Chinese patients improves
our current understanding of lung carcinogenesis, early diagnosis and personalized
therapy.
Type
Journal articleSubject
HumansCarcinoma, Squamous Cell
Lung Neoplasms
Sequence Analysis
Cell Adhesion
Mutation
Genes, Tumor Suppressor
China
Exome
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https://hdl.handle.net/10161/17962Published Version (Please cite this version)
10.1038/srep14237Publication Info
Li, Chenguang; Gao, Zhibo; Li, Fei; Li, Xiangchun; Sun, Yihua; Wang, Mengyun; ...
Wei, Qingyi (2015). Whole Exome Sequencing Identifies Frequent Somatic Mutations in Cell-Cell Adhesion
Genes in Chinese Patients with Lung Squamous Cell Carcinoma. Scientific reports, 5(1). pp. 14237. 10.1038/srep14237. Retrieved from https://hdl.handle.net/10161/17962.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Qingyi Wei
Professor in Population Health Sciences
Qingyi Wei, MD, PhD, Professor in the Department of Medicine, is Associate Director
for Cancer Control and Population Sciences, Co-leader of CCPS and Co-leader of Epidemiology
and Population Genomics (Focus Area 1). He is a professor of Medicine and an internationally
recognized epidemiologist focused on the molecular and genetic epidemiology of head
and neck cancers, lung cancer, and melanoma. His research focuses on biomarkers and
genetic determinants for the DNA repair deficient phenotype and

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