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Whole Exome Sequencing Identifies Frequent Somatic Mutations in Cell-Cell Adhesion Genes in Chinese Patients with Lung Squamous Cell Carcinoma.

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Date
2015-10-27
Authors
Li, Chenguang
Gao, Zhibo
Li, Fei
Li, Xiangchun
Sun, Yihua
Wang, Mengyun
Li, Dan
Wang, Rui
Li, Fuming
Fang, Rong
Pan, Yunjian
Luo, Xiaoyang
He, Jing
Zheng, Liangtao
Xia, Jufeng
Qiu, Lixin
He, Jun
Ye, Ting
Zhang, Ruoxin
He, Minghui
Zhu, Meiling
Hu, Haichuan
Shi, Tingyan
Zhou, Xiaoyan
Sun, Menghong
Tian, Shilin
Zhou, Yong
Wang, Qiaoxiu
Chen, Longyun
Yin, Guangliang
Lu, Jingya
Wu, Renhua
Guo, Guangwu
Li, Yingrui
Hu, Xueda
Li, Lin
Asan
Wang, Qin
Yin, Ye
Feng, Qiang
Wang, Bin
Wang, Hang
Wang, Mingbang
Yang, Xiaonan
Zhang, Xiuqing
Yang, Huanming
Jin, Li
Wang, Cun-Yu
Ji, Hongbin
Chen, Haiquan
Wang, Jun
Wei, Qingyi
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Abstract
Lung squamous cell carcinoma (SQCC) accounts for about 30% of all lung cancer cases. Understanding of mutational landscape for this subtype of lung cancer in Chinese patients is currently limited. We performed whole exome sequencing in samples from 100 patients with lung SQCCs to search for somatic mutations and the subsequent target capture sequencing in another 98 samples for validation. We identified 20 significantly mutated genes, including TP53, CDH10, NFE2L2 and PTEN. Pathways with frequently mutated genes included those of cell-cell adhesion/Wnt/Hippo in 76%, oxidative stress response in 21%, and phosphatidylinositol-3-OH kinase in 36% of the tested tumor samples. Mutations of Chromatin regulatory factor genes were identified at a lower frequency. In functional assays, we observed that knockdown of CDH10 promoted cell proliferation, soft-agar colony formation, cell migration and cell invasion, and overexpression of CDH10 inhibited cell proliferation. This mutational landscape of lung SQCC in Chinese patients improves our current understanding of lung carcinogenesis, early diagnosis and personalized therapy.
Type
Journal article
Subject
Humans
Carcinoma, Squamous Cell
Lung Neoplasms
Sequence Analysis
Cell Adhesion
Mutation
Genes, Tumor Suppressor
China
Exome
Permalink
https://hdl.handle.net/10161/17962
Published Version (Please cite this version)
10.1038/srep14237
Publication Info
Li, Chenguang; Gao, Zhibo; Li, Fei; Li, Xiangchun; Sun, Yihua; Wang, Mengyun; ... Wei, Qingyi (2015). Whole Exome Sequencing Identifies Frequent Somatic Mutations in Cell-Cell Adhesion Genes in Chinese Patients with Lung Squamous Cell Carcinoma. Scientific reports, 5(1). pp. 14237. 10.1038/srep14237. Retrieved from https://hdl.handle.net/10161/17962.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Wei

Qingyi Wei

Professor in Population Health Sciences
Qingyi Wei, MD, PhD, Professor in the Department of Medicine, is Associate Director for Cancer Control and Population Sciences, Co-leader of CCPS and Co-leader of Epidemiology and Population Genomics (Focus Area 1). He is a professor of Medicine and an internationally recognized epidemiologist focused on the molecular and genetic epidemiology of head and neck cancers, lung cancer, and melanoma. His research focuses on biomarkers and genetic determinants for the DNA repair deficient phenotype and
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