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Whole Exome Sequencing Identifies Frequent Somatic Mutations in Cell-Cell Adhesion Genes in Chinese Patients with Lung Squamous Cell Carcinoma.

dc.contributor.author Wei, Qingyi
dc.contributor.author Li, Chenguang
dc.contributor.author Gao, Zhibo
dc.contributor.author Li, Fei
dc.contributor.author Li, Xiangchun
dc.contributor.author Sun, Yihua
dc.contributor.author Wang, Mengyun
dc.contributor.author Li, Dan
dc.contributor.author Wang, Rui
dc.contributor.author Li, Fuming
dc.contributor.author Fang, Rong
dc.contributor.author Pan, Yunjian
dc.contributor.author Luo, Xiaoyang
dc.contributor.author He, Jing
dc.contributor.author Zheng, Liangtao
dc.contributor.author Xia, Jufeng
dc.contributor.author Qiu, Lixin
dc.contributor.author He, Jun
dc.contributor.author Ye, Ting
dc.contributor.author Zhang, Ruoxin
dc.contributor.author He, Minghui
dc.contributor.author Zhu, Meiling
dc.contributor.author Hu, Haichuan
dc.contributor.author Shi, Tingyan
dc.contributor.author Zhou, Xiaoyan
dc.contributor.author Sun, Menghong
dc.contributor.author Tian, Shilin
dc.contributor.author Zhou, Yong
dc.contributor.author Wang, Qiaoxiu
dc.contributor.author Chen, Longyun
dc.contributor.author Yin, Guangliang
dc.contributor.author Lu, Jingya
dc.contributor.author Wu, Renhua
dc.contributor.author Guo, Guangwu
dc.contributor.author Li, Yingrui
dc.contributor.author Hu, Xueda
dc.contributor.author Li, Lin
dc.contributor.author Wang, Qin
dc.contributor.author Yin, Ye
dc.contributor.author Feng, Qiang
dc.contributor.author Wang, Bin
dc.contributor.author Wang, Hang
dc.contributor.author Wang, Mingbang
dc.contributor.author Yang, Xiaonan
dc.contributor.author Zhang, Xiuqing
dc.contributor.author Yang, Huanming
dc.contributor.author Jin, Li
dc.contributor.author Wang, Cun-Yu
dc.contributor.author Ji, Hongbin
dc.contributor.author Chen, Haiquan
dc.contributor.author Wang, Jun
dc.date.accessioned 2019-02-01T15:02:21Z
dc.date.available 2019-02-01T15:02:21Z
dc.date.issued 2015-10-27
dc.identifier srep14237
dc.identifier.issn 2045-2322
dc.identifier.issn 2045-2322
dc.identifier.uri https://hdl.handle.net/10161/17962
dc.description.abstract Lung squamous cell carcinoma (SQCC) accounts for about 30% of all lung cancer cases. Understanding of mutational landscape for this subtype of lung cancer in Chinese patients is currently limited. We performed whole exome sequencing in samples from 100 patients with lung SQCCs to search for somatic mutations and the subsequent target capture sequencing in another 98 samples for validation. We identified 20 significantly mutated genes, including TP53, CDH10, NFE2L2 and PTEN. Pathways with frequently mutated genes included those of cell-cell adhesion/Wnt/Hippo in 76%, oxidative stress response in 21%, and phosphatidylinositol-3-OH kinase in 36% of the tested tumor samples. Mutations of Chromatin regulatory factor genes were identified at a lower frequency. In functional assays, we observed that knockdown of CDH10 promoted cell proliferation, soft-agar colony formation, cell migration and cell invasion, and overexpression of CDH10 inhibited cell proliferation. This mutational landscape of lung SQCC in Chinese patients improves our current understanding of lung carcinogenesis, early diagnosis and personalized therapy.
dc.language eng
dc.publisher Springer Nature
dc.relation.ispartof Scientific reports
dc.relation.isversionof 10.1038/srep14237
dc.subject Humans
dc.subject Carcinoma, Squamous Cell
dc.subject Lung Neoplasms
dc.subject Sequence Analysis
dc.subject Cell Adhesion
dc.subject Mutation
dc.subject Genes, Tumor Suppressor
dc.subject China
dc.subject Exome
dc.title Whole Exome Sequencing Identifies Frequent Somatic Mutations in Cell-Cell Adhesion Genes in Chinese Patients with Lung Squamous Cell Carcinoma.
dc.type Journal article
duke.contributor.id Wei, Qingyi|0632334
dc.date.updated 2019-02-01T15:02:19Z
pubs.begin-page 14237
pubs.issue 1
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Population Health Sciences
pubs.organisational-group Basic Science Departments
pubs.organisational-group Medicine, Medical Oncology
pubs.organisational-group Medicine
pubs.organisational-group Clinical Science Departments
pubs.publication-status Published
pubs.volume 5
duke.contributor.orcid Wei, Qingyi|0000-0002-3845-9445


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