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Serial dilution curve: a new method for analysis of reverse phase protein array data.

dc.contributor.author Wei, Qingyi
dc.contributor.author Zhang, Li
dc.contributor.author Mao, Li
dc.contributor.author Liu, Wenbin
dc.contributor.author Mills, Gordon B
dc.contributor.author Coombes, Kevin
dc.date.accessioned 2019-02-01T15:07:10Z
dc.date.available 2019-02-01T15:07:10Z
dc.date.issued 2009-03
dc.identifier btn663
dc.identifier.issn 1367-4803
dc.identifier.issn 1367-4811
dc.identifier.uri https://hdl.handle.net/10161/17970
dc.description.abstract Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples. In RPPA technology, the original samples are often diluted successively multiple times, forming dilution series to extend the dynamic range of the measurements and to increase confidence in quantitation. An RPPA experiment is equivalent to running multiple ELISA assays concurrently except that there is usually no known protein concentration from which one can construct a standard response curve. Here, we describe a new method called 'serial dilution curve for RPPA data analysis'. Compared with the existing methods, the new method has the advantage of using fewer parameters and offering a simple way of visualizing the raw data. We showed how the method can be used to examine data quality and to obtain robust quantification of protein concentrations.A computer program in R for using serial dilution curve for RPPA data analysis is freely available at http://odin.mdacc.tmc.edu/~zhangli/RPPA.
dc.language eng
dc.publisher Oxford University Press (OUP)
dc.relation.ispartof Bioinformatics (Oxford, England)
dc.relation.isversionof 10.1093/bioinformatics/btn663
dc.subject Proteins
dc.subject Protein Array Analysis
dc.subject Indicator Dilution Techniques
dc.subject Research Design
dc.subject Software
dc.title Serial dilution curve: a new method for analysis of reverse phase protein array data.
dc.type Journal article
dc.date.updated 2019-02-01T15:07:09Z
pubs.begin-page 650
pubs.end-page 654
pubs.issue 5
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Population Health Sciences
pubs.organisational-group Basic Science Departments
pubs.organisational-group Medicine, Medical Oncology
pubs.organisational-group Medicine
pubs.organisational-group Clinical Science Departments
pubs.publication-status Published
pubs.volume 25
duke.contributor.orcid Wei, Qingyi|0000-0002-3845-9445


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