Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.
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Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
DNA repair centers
gross chromosome rearrangements
pulsed field gel electrophoresis
replication fork stalling
sister chromatid recombination
sister repetitive sequences
site-specific chromosome breaks
toxic recombination intermediates
yeast artificial chromosome
Published Version (Please cite this version)10.15698/mic2019.01.664
Publication InfoPetes, Thomas; Klein, Hannah L; Bačinskaja, Giedrė; Che, Jun; Cheblal, Anais; Elango, Rajula; ... Malkova, Anna (2019). Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways. Microbial cell (Graz, Austria), 6(1). pp. 1-64. 10.15698/mic2019.01.664. Retrieved from https://hdl.handle.net/10161/18039.
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Minnie Geller Professor of Research in Genetics, in the School of Medicine
My lab is active in three somewhat related research areas: 1) the mechanism of mitotic recombination, 2) the genetic regulation of genome stability, and 3) genetic instability associated with interstitial telomeric sequences. Almost all of our studies are done using the yeast Saccharomyces cerevisiae. Mechanism of mitotic recombination Mitotic recombination, an important mechanism for the repair of DNA damage, is less well characterized than meiotic rec
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