Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.
Repository Usage Stats
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
DNA repair centers
gross chromosome rearrangements
pulsed field gel electrophoresis
replication fork stalling
sister chromatid recombination
sister repetitive sequences
site-specific chromosome breaks
toxic recombination intermediates
yeast artificial chromosome
Published Version (Please cite this version)10.15698/mic2019.01.664
Publication InfoKlein, Hannah L; Bačinskaja, Giedrė; Che, Jun; Cheblal, Anais; Elango, Rajula; Epshtein, Anastasiya; ... Malkova, Anna (2019). Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways. Microbial cell (Graz, Austria), 6(1). pp. 1-64. 10.15698/mic2019.01.664. Retrieved from https://hdl.handle.net/10161/18039.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
More InfoShow full item record
Mary Bernheim Distinguished Professor
My research focuses on the regulation of genetic stability and primarily uses budding yeast (Saccharomyces cerevisiae) as a model genetic system. The two primary research goals in the budding yeast system are (1) defining molecular structures and mechanisms of mitotic recombination intermediates and (2) understanding how and why transcription destabilizes the underlying DNA template. We also have initiated studies of mutagenesis in the pathogenic fungus Cryptococcus neof
Minnie Geller Distinguished Professor of Research in Genetics, in the School of Medicine
My lab is active in three somewhat related research areas: 1) the mechanism of mitotic recombination, 2) the genetic regulation of genome stability, and 3) genetic instability associated with interstitial telomeric sequences. Almost all of our studies are done using the yeast Saccharomyces cerevisiae. Mechanism of mitotic recombination Mitotic recombination, an important mechanism for the repair of DNA damage, is less well characterized than meiotic rec
Alphabetical list of authors with Scholars@Duke profiles.
Showing items related by title, author, creator, and subject.
Examination of Endogenous Rotund Expression and Function in Developing Drosophila Olfactory System Using CRISPR-Cas9-Mediated Protein Tagging. Li, Qingyun; Barish, Scott; Okuwa, Sumie; Volkan, Pelin C (G3 (Bethesda), 2015-10-23)The zinc-finger protein Rotund (Rn) plays a critical role in controlling the development of the fly olfactory system. However, little is known about its molecular function in vivo. Here, we added protein tags to the rn locus ...
Daaka, Y; Pitcher, JA; Richardson, M; Stoffel, RH; Robishaw, JD; Lefkowitz, RJ (Proc Natl Acad Sci U S A, 1997-03-18)The G protein-coupled receptor (GPCR) kinases (GRKs) phosphorylate and desensitize agonist-occupied GPCRs. GRK2-mediated receptor phosphorylation is preceded by the agonist-dependent membrane association of this enzyme. ...
Korunes, Katharine L (2019)Meiotic recombination creates genetic diversity by shuffling combinations of alleles across loci, yet alleles at neighboring loci often remain non-randomly associated. This non-random association is known as linkage-dise...