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    Increased predominance of HIV-1 CRF01_AE and its recombinants in the Philippines.

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    Date
    2019-01-24
    Authors
    Denny, Thomas
    Gao, Feng
    Chen, Yue
    Hora, Bhavna
    DeMarco, Todd
    Berba, Regina
    Register, Heidi
    Hood, Sylvia
    Carter, Meredith
    Stone, Mars
    Pappas, Andrea
    Sanchez, Ana M
    Busch, Michael
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    Abstract
    The growth rate of new HIV infections in the Philippines was the fastest of any countries in the Asia-Pacific region between 2010 and 2016. To date, HIV-1 subtyping results in the Philippines have been determined by characterizing only partial viral genome sequences. It is not known whether recombination occurs in the majority of unsequenced genome regions. Near-full-length genome (NFLG) sequences were obtained by amplifying two overlapping half genomes from plasma samples collected between 2015 and 2017 from 23 newly diagnosed infected individuals in the Philippines. Phylogenetic analysis showed that the newly characterized sequences were CRF01_AE (14), subtype B (3), CRF01/B recombinants (5) and a CRF01/CRF07/B recombinant (1). All 14 CRF01_AE formed a tight cluster, suggesting that they were derived from a single introduction. The time to the most recent common ancestor (tMRCA) for CRF01_AE in the Philippines was 1995 (1992-1998), about 10-15 years later than that of CRF01_AE in China and Thailand. All five CRF01/B recombinants showed distinct recombination patterns, suggesting ongoing recombination between the two predominant circulating viruses. The identification of partial CRF07_BC sequences in one CRF01/CRF07/B recombinant, not reported previously in the Philippines, indicated that CRF07_BC may have been recently introduced into that country from China, where CRF07_BC is prevalent. Our results show that the major epidemic strains may have shifted to an increased predominance of CRF01_AE and its recombinants, and that other genotypes such as CRF07_BC may have been introduced into the Philippines.
    Type
    Journal article
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    https://hdl.handle.net/10161/18041
    Published Version (Please cite this version)
    10.1099/jgv.0.001198
    Publication Info
    Denny, Thomas; Gao, Feng; Chen, Yue; Hora, Bhavna; DeMarco, Todd; Berba, Regina; ... Busch, Michael (2019). Increased predominance of HIV-1 CRF01_AE and its recombinants in the Philippines. The Journal of general virology. 10.1099/jgv.0.001198. Retrieved from https://hdl.handle.net/10161/18041.
    This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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    Scholars@Duke

    Denny

    Thomas Norton Denny

    Professor in Medicine
    Thomas N. Denny, MSc, M.Phil, is the Chief Operating Officer of the Duke Human Vaccine Institute (DHVI) and the Center for HIV/AIDS Vaccine Immunology (CHAVI), and a Professor of Medicine in the Department of Medicine at Duke University Medical Center. He is also an Affiliate Member of the Duke Global Health Institute. He has recently been appointed to the Duke University Fuqua School of Business Health Sector Advisory Council. Previously, he was an Associate Professor of Pathology, Laboratory M
    Gao

    Feng Gao

    Professor of Medicine
    Dr. Feng Gao is Professor of Medicine at Duke University. The Gao laboratory has a long-standing interest in elucidating the origins and evolution of human and simian inmmunodeficiency viruses (HIV and SIV), and in studying HIV/SIV gene function and pathogenic mechanisms from the evolutionary perspective. These studies have led to new strategies to better understand HIV origins,  biology, pathogenesis and drug resistance, and to design new AIDS vaccines.
    Alphabetical list of authors with Scholars@Duke profiles.
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