Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1 Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation?
Abstract
Monocyte chemoattractant protein-1 (MCP-1) overproduction from inflamed adipose tissue
is a major contributor to obesity-related metabolic syndromes. 3T3-L1 embryonic fibroblasts
were cultured and differentiated into adipocytes using an established protocol. Adipocytes
were treated with lipopolysaccharide (LPS) to induce inflammation and thus MCP-1 release.
At the same time, varying concentrations of chondroitin sulfate (CS) were added in
a physiologically relevant range (10-200 µg/mL) to determine its impact on MCP-1 release.
Chondroitin sulfate, a natural glycosaminoglycan of connective tissue including the
cartilage extracellular matrix, was chosen on the basis of our previous studies demonstrating
its anti-inflammatory effect on macrophages. Because the main action of MCP-1 is to
induce monocyte migration, cultured THP-1 monocytes were used to test whether CS at
the highest physiologically relevant concentration could inhibit cell migration induced
by human recombinant MCP-1. Chondroitin sulfate (100-200 µg/mL) inhibited MCP-1 release
from inflamed adipocytes in a dose-dependent manner (P < .01, 95% confidence interval
[CI]: -5.89 to -3.858 at 100 µg/mL and P < .001, 95% CI: -6.028 to -3.996 at 200 µg/mL)
but had no effect on MCP-1-driven chemotaxis of THP-1 monocytes. In summary, CS could
be expected to reduce macrophage infiltration into adipose tissue by reduction in
adipocyte expression and release of MCP-1 and as such might reduce adipose tissue
inflammation in response to pro-inflammatory stimuli such as LPS, now increasingly
recognized to be relevant in vivo.
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https://hdl.handle.net/10161/18051Published Version (Please cite this version)
10.1177/1177271917726964Publication Info
Stabler, Thomas V; Montell, Eulàlia; Vergés, Josep; Huebner, Janet L; & Kraus, Virginia
Byers (2017). Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1
Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation?. Biomarker insights, 12. pp. 1177271917726964. 10.1177/1177271917726964. Retrieved from https://hdl.handle.net/10161/18051.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Virginia Byers Kraus
Professor of Medicine
My special area of expertise is as a clinician scientist investigating osteoarthritis.
Osteoarthritis is the most common form of joint disease in man and its incidence increases
with age. It is a problem of increasing concern to the medical community due to the
increasing longevity of the population. Trained as a molecular biologist and a Rheumatologist,
I endeavor to study this disease from bedside to bench. The work in this laboratory
focuses on osteoarthritis and deals w

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