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Age-related changes in the cellular composition and epithelial organization of the mouse trachea.

dc.contributor.author Hogan, Brigid
dc.contributor.author Yu, Yen-Rei
dc.contributor.author Bowie, Emily
dc.contributor.author Wansleeben, Carolien
dc.contributor.author Hotten, Danielle F
dc.date.accessioned 2019-02-22T14:53:46Z
dc.date.available 2019-02-22T14:53:46Z
dc.date.issued 2014-01
dc.identifier PONE-D-14-03309
dc.identifier.issn 1932-6203
dc.identifier.issn 1932-6203
dc.identifier.uri https://hdl.handle.net/10161/18067
dc.description.abstract We report here senescent changes in the structure and organization of the mucociliary pseudostratified epithelium of the mouse trachea and main stem bronchi. We confirm previous reports of the gradual appearance of age-related, gland-like structures (ARGLS) in the submucosa, especially in the intercartilage regions and carina. Immunohistochemistry shows these structures contain ciliated and secretory cells and Krt5+ basal cells, but not the myoepithelial cells or ciliated ducts typical of normal submucosal glands. Data suggest they arise de novo by budding from the surface epithelium rather than by delayed growth of rudimentary or cryptic submucosal glands. In old mice the surface epithelium contains fewer cells per unit length than in young mice and the proportion of Krt5+, p63+ basal cells is reduced in both males and females. However, there appears to be no significant difference in the ability of basal stem cells isolated from individual young and old mice to form clonal tracheospheres in culture or in the ability of the epithelium to repair after damage by inhaled sulfur dioxide. Gene expression analysis by Affymetrix microarray and quantitative PCR, as well as immunohistochemistry and flow sorting studies, are consistent with low-grade chronic inflammation in the tracheas of old versus young mice and an increase in the number of immune cells. The significance of these changes for ARGL formation are not clear since several treatments that induce acute inflammation in young mice did not result in budding of the surface epithelium.
dc.language eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PloS one
dc.relation.isversionof 10.1371/journal.pone.0093496
dc.subject Bronchi
dc.subject Respiratory Mucosa
dc.subject Trachea
dc.subject Cells, Cultured
dc.subject Spheroids, Cellular
dc.subject Epithelial Cells
dc.subject Stem Cells
dc.subject Animals
dc.subject Mice, Knockout
dc.subject Mice
dc.subject Trans-Activators
dc.subject Phosphoproteins
dc.subject Cell Division
dc.subject Cell Differentiation
dc.subject Gene Expression
dc.subject Aging
dc.subject Female
dc.subject Male
dc.subject Myeloid Differentiation Factor 88
dc.subject Keratin-15
dc.title Age-related changes in the cellular composition and epithelial organization of the mouse trachea.
dc.type Journal article
dc.date.updated 2019-02-22T14:53:41Z
pubs.begin-page e93496
pubs.issue 3
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Cell Biology
pubs.organisational-group Basic Science Departments
pubs.organisational-group Pediatrics
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Medicine, Pulmonary, Allergy, and Critical Care Medicine
pubs.organisational-group Medicine
pubs.organisational-group Student
pubs.publication-status Published
pubs.volume 9


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