Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model
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© 2018, Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature. Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a promising type of surface-enhanced Raman scattering (SERS) nanosensor implanted in the skin of a large animal model (pig). The in vivo nanosensor used in this study involves the “inverse molecular sentinel” detection scheme using plasmonics-active nanostars, which have tunable absorption bands in the near infrared region of the “tissue optical window”, rendering them efficient as an optical sensing platform for in vivo optical detection. Ex vivo measurements were also performed using human skin grafts to demonstrate the detection of SERS nanosensors through tissue. In this study, a new core–shell nanorattle probe with Raman reporters trapped between the core and shell was utilized as an internal standard system for self-calibration. These results illustrate the usefulness and translational potential of the SERS nanosensor for in vivo biosensing. [Figure not available: see fulltext.].
SubjectScience & Technology
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Science & Technology - Other Topics
surface-enhanced Raman scattering (SERS)
in vivo sensing
COATED GOLD NANOSTARS
Published Version (Please cite this version)10.1007/s12274-018-1982-3
Publication InfoWang, HN; Register, JK; Fales, AM; Gandra, N; Cho, EH; Boico, A; ... Vo-Dinh, T (2018). Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model. Nano Research, 11(8). pp. 4005-4016. 10.1007/s12274-018-1982-3. Retrieved from https://hdl.handle.net/10161/18477.
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Associate Professor of Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors, and long term improvement of medical device implantation. My basic research interests are in the area of physiological mechanisms of optimizing substrate transport to tissue. This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular, cellular, ultrastructural, and molecular level. The current projects include: 1) control of blood flow and flow distribu
Associate Professor of Radiation Oncology
Greg Palmer obtained his B.S. in Biomedical Engineering from Marquette University in 2000, after which he obtained his Ph.D. in BME from the University of Wisconsin, Madison. He is currently an Associate Professor in the Department of Radiation Oncology, Cancer Biology Division at Duke University Medical Center. His primary research focus has been identifying and exploiting the changes in absorption, scattering, and fluorescence properties of tissue associated with cancer progression and therape
R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering
Dr. Tuan Vo-Dinh is R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering, Professor of Chemistry, and Director of The Fitzpatrick Institute for Photonics.Dr. Vo-Dinh’s research activities and interests involve biophotonics, nanophotonics, plasmonics, laser-excited luminescence spectroscopy, room temperature phosphorimetry, synchronous luminescence spectroscopy, surface-enhanced Raman spectroscopy, field environmental instrumentation, fiberoptics s
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