Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model
Abstract
© 2018, Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer
Nature. Although nanotechnology has led to important advances in in vitro diagnostics,
the development of nanosensors for in vivo detection remains very challenging. Here,
we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets
using a promising type of surface-enhanced Raman scattering (SERS) nanosensor implanted
in the skin of a large animal model (pig). The in vivo nanosensor used in this study
involves the “inverse molecular sentinel” detection scheme using plasmonics-active
nanostars, which have tunable absorption bands in the near infrared region of the
“tissue optical window”, rendering them efficient as an optical sensing platform for
in vivo optical detection. Ex vivo measurements were also performed using human skin
grafts to demonstrate the detection of SERS nanosensors through tissue. In this study,
a new core–shell nanorattle probe with Raman reporters trapped between the core and
shell was utilized as an internal standard system for self-calibration. These results
illustrate the usefulness and translational potential of the SERS nanosensor for in
vivo biosensing. [Figure not available: see fulltext.].
Type
Journal articleSubject
Science & TechnologyPhysical Sciences
Technology
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
nanosensor
nanoprobes
plasmonics
nanostar
surface-enhanced Raman scattering (SERS)
in vivo sensing
PHASE-CHANGE MATERIALS
COATED GOLD NANOSTARS
SERS DETECTION
CANCER-DETECTION
CONTROLLED-RELEASE
NANOPROBES
SPECTROSCOPY
BIOMARKERS
THERAPY
DNA
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https://hdl.handle.net/10161/18477Published Version (Please cite this version)
10.1007/s12274-018-1982-3Publication Info
Wang, HN; Register, JK; Fales, AM; Gandra, N; Cho, EH; Boico, A; ... Vo-Dinh, T (2018). Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid
targets in a large animal model. Nano Research, 11(8). pp. 4005-4016. 10.1007/s12274-018-1982-3. Retrieved from https://hdl.handle.net/10161/18477.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Bruce Klitzman
Associate Professor Emeritus in Surgery
Our overriding interests are in the fields of tissue engineering, wound healing, biosensors,
and long term improvement of medical device implantation. My basic research interests
are in the area of physiological mechanisms of optimizing substrate transport to tissue.
This broad topic covers studies on a whole animal, whole organ, hemorheological, microvascular,
cellular, ultrastructural, and molecular level. The current projects include:
1) control of blood flow and flow distribu
Gregory M. Palmer
Associate Professor of Radiation Oncology
Greg Palmer obtained his B.S. in Biomedical Engineering from Marquette University
in 2000, after which he obtained his Ph.D. in BME from the University of Wisconsin,
Madison. He is currently an Associate Professor in the Department of Radiation Oncology,
Cancer Biology Division at Duke University Medical Center. His primary research focus
has been identifying and exploiting the changes in absorption, scattering, and fluorescence
properties of tissue associated with cancer progression and therape
Tuan Vo-Dinh
R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical Engineering
Dr. Tuan Vo-Dinh is R. Eugene and Susie E. Goodson Distinguished Professor of Biomedical
Engineering, Professor of Chemistry, and Director of The Fitzpatrick Institute for
Photonics.
Dr. Vo-Dinh’s research activities and interests involve biophotonics, nanophotonics,
plasmonics, laser-excited luminescence spectroscopy, room temperature phosphorimetry,
synchronous luminescence spectroscopy, and surface-enhanced Raman spectroscopy for
multi-modality bioimaging, and theranostics (d
Alphabetical list of authors with Scholars@Duke profiles.

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