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Characterization of the Foreign Body Response to Common Surgical Biomaterials in a Murine Model. Ibrahim, Mohamed Bond, Jennifer Medina, Manuel A Chen, Lei Quiles, Carlos Kokosis, George Bashirov, Latif Klitzman, Bruce Levinson, Howard 2019-05-01T13:51:01Z 2019-05-01T13:51:01Z 2017-11
dc.identifier.issn 0930-343X
dc.identifier.issn 1435-0130
dc.description.abstract BACKGROUND:Implanted biomaterials are subject to a significant reaction from the host, known as the foreign body response (FBR). We quantified the FBR to five materials following subcutaneous implantation in mice. MATERIALS AND METHODS:Polyvinyl alcohol (PVA) and silicone sheets are considered highly biocompatible biomaterials and were cut into 8mm-diameter disks. Expanded PTFE (ePTFE)and polypropylene are also widely used biocompatible biomaterials and were cut into 2cm-long cylinders. Cotton was selected as a negative control material that would invoke an intense FBR, was cut into disks and implanted. The implants were inserted subcutaneously into female C57BL/6 mice. On post-implantation days 14, 30, 60, 90 and 180, implants were retrieved. Cellularity was assessed with DAPI stain, collagen with Masson's trichrome stain. mast cells with toluidine-blue, macrophages with F4/80 immunohistochemical-stain, and capsular thickness and foreign body giant cells with hematoxylin & eosin. RESULTS:DAPI revealed a significantly increased cellularity in both PVA andsilicone, and ePTFE had the lowest cell density. Silicone showed the lowest cellularity at d14 and d90 whereas ePTFE showed the lowest cellularity at days 30, 60, and 180. Masson's trichrome staining demonstrated no apparent difference in collagen. Toluidine blue showed no differences in mast cells. There were, however, fewer macrophages associated with ePTFE. On d14, PVA had highest number of macrophages, whereas polypropylene had the highest number at all time points after d14. Giant cells increased earlier and gradually decreased later. On d90, PVA exhibited a significantly increased number of giant cells compared to polypropylene and silicone. Silicone consistently formed the thinnest capsule throughout all time points. On d14, cotton had formed the thickest capsule. On d30 polypropylenehas formed thickest capsule and on days 60, 90 and 180, PVA had formed thickest capsule. CONCLUSION:These data reveal differences in capsule thickness and cellular response in an implant-related manor, indicating that fibrotic reactions to biomaterials are implant specific and should be carefully considered when performing studies on fibrosis when biomaterials are being used.
dc.language eng
dc.publisher Springer Science and Business Media LLC
dc.relation.ispartof European journal of plastic surgery
dc.relation.isversionof 10.1007/s00238-017-1308-9
dc.subject PVA
dc.subject Prolene
dc.subject biocompatibility
dc.subject ePTFE
dc.subject fibrosis
dc.subject implants
dc.subject polypropylene
dc.title Characterization of the Foreign Body Response to Common Surgical Biomaterials in a Murine Model.
dc.type Journal article Klitzman, Bruce|0017727 Levinson, Howard|0373516 2019-05-01T13:51:00Z
pubs.begin-page 383
pubs.end-page 392
pubs.issue 5
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group Cell Biology
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke Innovation & Entrepreneurship
pubs.organisational-group Initiatives
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Surgery, Plastic, Maxillofacial, and Oral Surgery
pubs.organisational-group Surgery
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Dermatology
pubs.organisational-group Pathology
pubs.publication-status Published
pubs.volume 40

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