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Associations between expression levels of nucleotide excision repair proteins in lymphoblastoid cells and risk of squamous cell carcinoma of the head and neck.

dc.contributor.author Troy, Jesse
dc.contributor.author Lee, Walter
dc.contributor.author Liu, Zhensheng
dc.contributor.author Wei, Qingyi
dc.contributor.author Han, Peng
dc.contributor.author Liu, Hongliang
dc.contributor.author Shi, Qiong
dc.contributor.author Zevallos, Jose P
dc.contributor.author Li, Guojun
dc.contributor.author Sturgis, Erich M
dc.date.accessioned 2019-05-01T18:30:51Z
dc.date.available 2019-05-01T18:30:51Z
dc.date.issued 2018-06
dc.identifier.issn 0899-1987
dc.identifier.issn 1098-2744
dc.identifier.uri https://hdl.handle.net/10161/18510
dc.description.abstract Squamous cell carcinoma of head and neck (SCCHN) is one of the most common malignancies worldwide, and nucleotide excision repair (NER) is involved in SCCHN susceptibility. In this analysis of 349 newly diagnosed SCCHN patients and 295 cancer-free controls, we investigated whether expression levels of eight core NER proteins were associated with risk of SCCHN. We quantified NER protein expression levels in cultured peripheral lymphocytes using a reverse-phase protein microarray. Compared with the controls, SCCHN patients had statistically significantly lower expression levels of ERCC3 and XPA (P = 0.001 and 0.001, respectively). After dividing the subjects by controls' median values of expression levels, we found a dose-dependent association between an increased risk of SCCHN and low expression levels of ERCC3 (adjusted OR, 1.75, and 95% CI: 1.26-2.42; Ptrend  = 0.008) and XPA (adjusted OR, 1.88; 95% CI, 1.35-2.60; Ptrend  = 0.001). We also identified a significant multiplicative interaction between smoking status and ERCC3 expression levels (P = 0.014). Finally, after integrating demographic and clinical variables, we found that the addition of ERCC3 and XPA expression levels to the model significantly improved the sensitivity of the expanded model on SCCHN risk. In conclusion, reduced protein expression levels of ERCC3 and XPA were associated with an increased risk of SCCHN. However, these results need to be confirmed in additional large studies.
dc.language eng
dc.publisher Wiley
dc.relation.ispartof Molecular carcinogenesis
dc.relation.isversionof 10.1002/mc.22801
dc.subject Lymphocytes
dc.subject Cells, Cultured
dc.subject Humans
dc.subject Carcinoma, Squamous Cell
dc.subject Head and Neck Neoplasms
dc.subject DNA Helicases
dc.subject DNA-Binding Proteins
dc.subject Risk Assessment
dc.subject Risk Factors
dc.subject Adult
dc.subject Aged
dc.subject Aged, 80 and over
dc.subject Middle Aged
dc.subject Female
dc.subject Male
dc.subject Xeroderma Pigmentosum Group A Protein
dc.subject Young Adult
dc.title Associations between expression levels of nucleotide excision repair proteins in lymphoblastoid cells and risk of squamous cell carcinoma of the head and neck.
dc.type Journal article
dc.date.updated 2019-05-01T18:30:51Z
pubs.begin-page 784
pubs.end-page 793
pubs.issue 6
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Pediatrics, Blood and Marrow Transplantation
pubs.organisational-group Pediatrics
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Radiation Oncology
pubs.organisational-group Surgery, Head and Neck Surgery and Communication Sciences
pubs.organisational-group Surgery
pubs.organisational-group Staff
pubs.organisational-group Population Health Sciences
pubs.organisational-group Basic Science Departments
pubs.organisational-group Medicine, Medical Oncology
pubs.organisational-group Medicine
pubs.publication-status Published
pubs.volume 57
duke.contributor.orcid Troy, Jesse|0000-0001-5410-8146
duke.contributor.orcid Lee, Walter|0000-0002-5487-3603
duke.contributor.orcid Wei, Qingyi|0000-0002-3845-9445


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