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Kinematic and dynamic gait compensations in a rat model of lumbar radiculopathy and the effects of tumor necrosis factor-alpha antagonism.

dc.contributor.author Schmitt, Daniel
dc.contributor.author Richardson, William
dc.contributor.author Setton, Lori
dc.contributor.author Gabr, Mostafa
dc.contributor.author Sinclair, S Michael
dc.date.accessioned 2019-06-01T09:46:32Z
dc.date.available 2019-06-01T09:46:32Z
dc.date.issued 2011-08-26
dc.identifier ar3451
dc.identifier.issn 1478-6354
dc.identifier.issn 1478-6362
dc.identifier.uri https://hdl.handle.net/10161/18593
dc.description.abstract Tumor necrosis factor-α (TNFα) has received significant attention as a mediator of lumbar radiculopathy, with interest in TNF antagonism to treat radiculopathy. Prior studies have demonstrated that TNF antagonists can attenuate heightened nociception resulting from lumbar radiculopathy in the preclinical model. Less is known about the potential impact of TNF antagonism on gait compensations, despite being of clinical relevance. In this study, we expand on previous descriptions of gait compensations resulting from lumbar radiculopathy in the rat and describe the ability of local TNF antagonism to prevent the development of gait compensations, altered weight bearing, and heightened nociception.Eighteen male Sprague-Dawley rats were investigated for mechanical sensitivity, weight-bearing, and gait pre- and post-operatively. For surgery, tail nucleus pulposus (NP) tissue was collected and the right L5 dorsal root ganglion (DRG) was exposed (Day 0). In sham animals, NP tissue was discarded (n = 6); for experimental animals, autologous NP was placed on the DRG with or without 20 μg of soluble TNF receptor type II (sTNFRII, n = 6 per group). Spatiotemporal gait characteristics (open arena) and mechanical sensitivity (von Frey filaments) were assessed on post-operative Day 5; gait dynamics (force plate arena) and weight-bearing (incapacitance meter) were assessed on post-operative Day 6.High-speed gait characterization revealed animals with NP alone had a 5% decrease in stance time on their affected limbs on Day 5 (P ≤0.032). Ground reaction force analysis on Day 6 aligned with temporal changes observed on Day 5, with vertical impulse reduced in the affected limb of animals with NP alone (area under the vertical force-time curve, P <0.02). Concordant with gait, animals with NP alone also had some evidence of affected limb mechanical allodynia on Day 5 (P = 0.08) and reduced weight-bearing on the affected limb on Day 6 (P <0.05). Delivery of sTNFRII at the time of NP placement ameliorated signs of mechanical hypersensitivity, imbalanced weight distribution, and gait compensations (P <0.1).Our data indicate gait characterization has value for describing early limb dysfunctions in pre-clinical models of lumbar radiculopathy. Furthermore, TNF antagonism prevented the development of gait compensations subsequent to lumbar radiculopathy in our model.
dc.language eng
dc.publisher Springer Nature
dc.relation.ispartof Arthritis research & therapy
dc.relation.isversionof 10.1186/ar3451
dc.subject Lumbosacral Region
dc.subject Animals
dc.subject Rats
dc.subject Rats, Sprague-Dawley
dc.subject Gait Disorders, Neurologic
dc.subject Hyperalgesia
dc.subject Radiculopathy
dc.subject Disease Models, Animal
dc.subject Tumor Necrosis Factor-alpha
dc.subject Receptors, Tumor Necrosis Factor, Type II
dc.subject Gait
dc.subject Adaptation, Physiological
dc.subject Weight-Bearing
dc.subject Male
dc.subject Biomechanical Phenomena
dc.title Kinematic and dynamic gait compensations in a rat model of lumbar radiculopathy and the effects of tumor necrosis factor-alpha antagonism.
dc.type Journal article
dc.date.updated 2019-06-01T09:46:29Z
pubs.begin-page R137
pubs.issue 4
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Orthopaedics
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Neurosurgery
pubs.organisational-group Trinity College of Arts & Sciences
pubs.organisational-group Evolutionary Anthropology
pubs.organisational-group Duke Science & Society
pubs.organisational-group Initiatives
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Faculty
pubs.organisational-group Staff
pubs.publication-status Published
pubs.volume 13
duke.contributor.orcid Gabr, Mostafa|0000-0003-2058-2098


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