Skip to main content
Duke University Libraries
DukeSpace Scholarship by Duke Authors
  • Login
  • Ask
  • Menu
  • Login
  • Ask a Librarian
  • Search & Find
  • Using the Library
  • Research Support
  • Course Support
  • Libraries
  • About
View Item 
  •   DukeSpace
  • Theses and Dissertations
  • Duke Dissertations
  • View Item
  •   DukeSpace
  • Theses and Dissertations
  • Duke Dissertations
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Molecular and Biofunctional Modification of Conformal POEGMA Bottlebrush Coatings and Applications Toward In Vitro Diagnostics

Thumbnail
View / Download
12.5 Mb
Date
2019
Author
Joh, Daniel
Advisor
Chilkoti, Ashutosh
Repository Usage Stats
195
views
58
downloads
Abstract

In vitro diagnostic assays (IVDs) play a crucial role in modern biomedical research and clinical decision-making. The overall performance and cost of IVDs depends considerably on the quality of the assay surface being used and the steps/reagents required to complete the assay. Synthetic “bottlebrush” polymers have garnered considerable attention as molecularly defined and tunable biointerfacial coatings for a diverse set of biomedical applications; in particular, their bio-inertness, ability to eliminate nonspecific binding events and stabilize biorecognition elements make them attractive candidates as biosensing surfaces. This dissertation describes the use of “nonfouling” (protein- and cell-resistant) nanoscale polymer bottlebrush films derived from poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) for IVD applications. POEGMA coatings are grown from planar surfaces by surface-initiated atom transfer radical polymerization (SI-ATRP), and then undergo biofunctional modification with biorecognition elements to target specific analytes of interest. This strategy is used to assemble low-cost, “point of care” diagnostic tests which exhibit performance comparable to those used in central laboratories. Further, molecular modification of bottlebrush architecture is used as a controllable design parameter for fine-tuning the physiochemical properties and biological behavior of these surfaces. Taken together, these studies highlight the potential for using POEGMA bottlebrush films as biointerfacial coatings for next-generation IVDs and medical devices.

Description
Dissertation
Type
Dissertation
Department
Biomedical Engineering
Subject
Bioengineering
Permalink
https://hdl.handle.net/10161/18642
Citation
Joh, Daniel (2019). Molecular and Biofunctional Modification of Conformal POEGMA Bottlebrush Coatings and Applications Toward In Vitro Diagnostics. Dissertation, Duke University. Retrieved from https://hdl.handle.net/10161/18642.
Collections
  • Duke Dissertations
More Info
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Rights for Collection: Duke Dissertations


Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info

Make Your Work Available Here

How to Deposit

Browse

All of DukeSpaceCommunities & CollectionsAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit DateThis CollectionAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit Date

My Account

LoginRegister

Statistics

View Usage Statistics
Duke University Libraries

Contact Us

411 Chapel Drive
Durham, NC 27708
(919) 660-5870
Perkins Library Service Desk

Digital Repositories at Duke

  • Report a problem with the repositories
  • About digital repositories at Duke
  • Accessibility Policy
  • Deaccession and DMCA Takedown Policy

TwitterFacebookYouTubeFlickrInstagramBlogs

Sign Up for Our Newsletter
  • Re-use & Attribution / Privacy
  • Harmful Language Statement
  • Support the Libraries
Duke University