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Molecular and Biofunctional Modification of Conformal POEGMA Bottlebrush Coatings and Applications Toward In Vitro Diagnostics

dc.contributor.advisor Chilkoti, Ashutosh
dc.contributor.author Joh, Daniel
dc.date.accessioned 2019-06-07T19:47:54Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10161/18642
dc.description Dissertation
dc.description.abstract <p>In vitro diagnostic assays (IVDs) play a crucial role in modern biomedical research and clinical decision-making. The overall performance and cost of IVDs depends considerably on the quality of the assay surface being used and the steps/reagents required to complete the assay. Synthetic “bottlebrush” polymers have garnered considerable attention as molecularly defined and tunable biointerfacial coatings for a diverse set of biomedical applications; in particular, their bio-inertness, ability to eliminate nonspecific binding events and stabilize biorecognition elements make them attractive candidates as biosensing surfaces. This dissertation describes the use of “nonfouling” (protein- and cell-resistant) nanoscale polymer bottlebrush films derived from poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) for IVD applications. POEGMA coatings are grown from planar surfaces by surface-initiated atom transfer radical polymerization (SI-ATRP), and then undergo biofunctional modification with biorecognition elements to target specific analytes of interest. This strategy is used to assemble low-cost, “point of care” diagnostic tests which exhibit performance comparable to those used in central laboratories. Further, molecular modification of bottlebrush architecture is used as a controllable design parameter for fine-tuning the physiochemical properties and biological behavior of these surfaces. Taken together, these studies highlight the potential for using POEGMA bottlebrush films as biointerfacial coatings for next-generation IVDs and medical devices.</p>
dc.subject Bioengineering
dc.title Molecular and Biofunctional Modification of Conformal POEGMA Bottlebrush Coatings and Applications Toward In Vitro Diagnostics
dc.type Dissertation
dc.department Biomedical Engineering
duke.embargo.months 23
duke.embargo.release 2021-05-21T00:00:00Z


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