ALERT: This system is being upgraded on Tuesday December 12. It will not be available for use for several hours that day while the upgrade is in progress. Deposits to DukeSpace will be disabled on Monday December 11, so no new items are to be added to the repository while the upgrade is in progress. Everything should be back to normal by the end of day, December 12.

Show simple item record

Interactions between oxidative stress and insulin/IGF-1 signaling for starvation resistance in Caenorhabditis elegans

dc.contributor.advisor Baugh, Ryan Jiao, Megan 2019-06-08T17:02:11Z 2019-06-08T17:02:11Z 2019-04-22
dc.description.abstract Reactive oxygen species (ROS) are a natural byproduct of metabolism with roles in cell signaling and homeostasis but also generate oxidative stress. Past research demonstrates that ROS are a major factor in pathological conditions and the aging process in Caenorhabditis elegans and other organisms. Additionally, transcription factor gene daf-16 from the insulin/IGF-1 signaling (IIS) pathway is thought to help manage oxidative stress to mitigate such consequences, which may be partly due to endogenous antioxidant genes downstream of it. Furthermore, exogenous antioxidant drugs such as N-acetylcysteine (NAC) have been found to extend mean and maximum survival time in C. elegans under a variety of conditions, including exposure to oxidative stress, high heat, and UV radiation. However, their effects on starvation resistance have not yet been examined. To uncover how the IIS pathway interacts with ROS and antioxidants in C. elegans, we performed assays for two measures of starvation resistance: starvation survival and growth rate following starvation, which enabled us to investigate how the presence and absence of ROS impacted the starvation recovery process. We demonstrated that NAC can significantly increase and decrease survival in wild-type worms in a dose-dependent manner. Additionally, NAC also increased worm length, a metric of their growth rate. In contrast, daf-16 mutants exposed to NAC had decreased size and survival. Moreover, mutating endogenous antioxidant genes downstream of daf-16 did not cause a significant decrease in worm survival. These complex interactions between IIS and NAC suggest that genotype may position worms at different baselines on a hormesis curve for antioxidants and consequently alter their sensitivity to ROS quenching.
dc.language.iso en_US
dc.subject Caenorhabditis elegans
dc.subject insulin/IGF-1 signaling
dc.subject starvation resistance
dc.subject antioxidants
dc.subject hormesis
dc.subject aging
dc.title Interactions between oxidative stress and insulin/IGF-1 signaling for starvation resistance in Caenorhabditis elegans
dc.type Honors thesis
dc.department Biology
duke.embargo.months 0

Files in this item


This item appears in the following Collection(s)

Show simple item record