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Meniscus-Derived Matrix Scaffolds Promote the Integrative Repair of Meniscal Defects.

dc.contributor.author Weinberg, Joe
dc.contributor.author McNulty, Amy
dc.contributor.author Ruprecht, Jacob C
dc.contributor.author Waanders, Taylor D
dc.contributor.author Rowland, Christopher R
dc.contributor.author Nishimuta, James F
dc.contributor.author Glass, Katherine A
dc.contributor.author Stencel, Jennifer
dc.contributor.author DeFrate, Louis E
dc.contributor.author Guilak, Farshid
dc.date.accessioned 2019-07-01T13:14:42Z
dc.date.available 2019-07-01T13:14:42Z
dc.date.issued 2019-06-18
dc.identifier 10.1038/s41598-019-44855-3
dc.identifier.issn 2045-2322
dc.identifier.issn 2045-2322
dc.identifier.uri https://hdl.handle.net/10161/19046
dc.description.abstract Meniscal tears have a poor healing capacity, and damage to the meniscus is associated with significant pain, disability, and progressive degenerative changes in the knee joint that lead to osteoarthritis. Therefore, strategies to promote meniscus repair and improve meniscus function are needed. The objective of this study was to generate porcine meniscus-derived matrix (MDM) scaffolds and test their effectiveness in promoting meniscus repair via migration of endogenous meniscus cells from the surrounding meniscus or exogenously seeded human bone marrow-derived mesenchymal stem cells (MSCs). Both endogenous meniscal cells and MSCs infiltrated the MDM scaffolds. In the absence of exogenous cells, the 8% MDM scaffolds promoted the integrative repair of an in vitro meniscal defect. Dehydrothermal crosslinking and concentration of the MDM influenced the biochemical content and shear strength of repair, demonstrating that the MDM can be tailored to promote tissue repair. These findings indicate that native meniscus cells can enhance meniscus healing if a scaffold is provided that promotes cellular infiltration and tissue growth. The high affinity of cells for the MDM and the ability to remodel the scaffold reveals the potential of MDM to integrate with native meniscal tissue to promote long-term repair without necessarily requiring exogenous cells.
dc.language eng
dc.publisher Springer Science and Business Media LLC
dc.relation.ispartof Scientific reports
dc.relation.isversionof 10.1038/s41598-019-44855-3
dc.title Meniscus-Derived Matrix Scaffolds Promote the Integrative Repair of Meniscal Defects.
dc.type Journal article
duke.contributor.id Weinberg, Joe|0115187
duke.contributor.id McNulty, Amy|0226708
dc.date.updated 2019-07-01T13:14:40Z
pubs.begin-page 8719
pubs.issue 1
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Immunology
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke Global Health Institute
pubs.organisational-group University Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Obstetrics and Gynecology
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Medicine, Hematology
pubs.organisational-group Medicine
pubs.publication-status Published
pubs.volume 9


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