dc.contributor.author |
Ruprecht, Jacob C |
|
dc.contributor.author |
Waanders, Taylor D |
|
dc.contributor.author |
Rowland, Christopher R |
|
dc.contributor.author |
Nishimuta, James F |
|
dc.contributor.author |
Glass, Katherine A |
|
dc.contributor.author |
Stencel, Jennifer |
|
dc.contributor.author |
DeFrate, Louis E |
|
dc.contributor.author |
Guilak, Farshid |
|
dc.contributor.author |
Weinberg, J Brice |
|
dc.contributor.author |
McNulty, Amy L |
|
dc.date.accessioned |
2019-07-01T13:14:42Z |
|
dc.date.available |
2019-07-01T13:14:42Z |
|
dc.date.issued |
2019-06-18 |
|
dc.identifier |
10.1038/s41598-019-44855-3 |
|
dc.identifier.issn |
2045-2322 |
|
dc.identifier.issn |
2045-2322 |
|
dc.identifier.uri |
https://hdl.handle.net/10161/19046 |
|
dc.description.abstract |
Meniscal tears have a poor healing capacity, and damage to the meniscus is associated
with significant pain, disability, and progressive degenerative changes in the knee
joint that lead to osteoarthritis. Therefore, strategies to promote meniscus repair
and improve meniscus function are needed. The objective of this study was to generate
porcine meniscus-derived matrix (MDM) scaffolds and test their effectiveness in promoting
meniscus repair via migration of endogenous meniscus cells from the surrounding meniscus
or exogenously seeded human bone marrow-derived mesenchymal stem cells (MSCs). Both
endogenous meniscal cells and MSCs infiltrated the MDM scaffolds. In the absence of
exogenous cells, the 8% MDM scaffolds promoted the integrative repair of an in vitro
meniscal defect. Dehydrothermal crosslinking and concentration of the MDM influenced
the biochemical content and shear strength of repair, demonstrating that the MDM can
be tailored to promote tissue repair. These findings indicate that native meniscus
cells can enhance meniscus healing if a scaffold is provided that promotes cellular
infiltration and tissue growth. The high affinity of cells for the MDM and the ability
to remodel the scaffold reveals the potential of MDM to integrate with native meniscal
tissue to promote long-term repair without necessarily requiring exogenous cells.
|
|
dc.language |
eng |
|
dc.publisher |
Springer Science and Business Media LLC |
|
dc.relation.ispartof |
Scientific reports |
|
dc.relation.isversionof |
10.1038/s41598-019-44855-3 |
|
dc.title |
Meniscus-Derived Matrix Scaffolds Promote the Integrative Repair of Meniscal Defects. |
|
dc.type |
Journal article |
|
duke.contributor.id |
DeFrate, Louis E|0401643 |
|
duke.contributor.id |
Guilak, Farshid|0115558 |
|
duke.contributor.id |
Weinberg, J Brice|0115187 |
|
duke.contributor.id |
McNulty, Amy L|0226708 |
|
dc.date.updated |
2019-07-01T13:14:40Z |
|
pubs.begin-page |
8719 |
|
pubs.issue |
1 |
|
pubs.organisational-group |
School of Medicine |
|
pubs.organisational-group |
Duke |
|
pubs.organisational-group |
Duke Cancer Institute |
|
pubs.organisational-group |
Institutes and Centers |
|
pubs.organisational-group |
Immunology |
|
pubs.organisational-group |
Basic Science Departments |
|
pubs.organisational-group |
Duke Global Health Institute |
|
pubs.organisational-group |
University Institutes and Centers |
|
pubs.organisational-group |
Institutes and Provost's Academic Units |
|
pubs.organisational-group |
Obstetrics and Gynecology |
|
pubs.organisational-group |
Clinical Science Departments |
|
pubs.organisational-group |
Medicine, Hematology |
|
pubs.organisational-group |
Medicine |
|
pubs.publication-status |
Published |
|
pubs.volume |
9 |
|
duke.contributor.orcid |
Weinberg, J Brice|0000-0003-4052-5576 |
|