Use of biological detection methods to assess dioxin-like compounds in sediments of Bohai Bay, China.
Abstract
Bohai Bay, in the western region of northeastern China's Bohai Sea, receives water
from large rivers containing various pollutants including dioxin-like compounds (DLCs).
This study used the established zebrafish (Danio rerio) model, its known developmental
toxicity endpoints and sensitive molecular analyses to evaluate sediments near and
around an industrial effluent site in Bohai Bay. The primary objective was to assess
the efficacy of rapid biological detection methods as an addition to chemical analyses.
Embryos were exposed to various concentrations of sediment extracts as well as a 2,
3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) positive control. Exposure to sediment
extract nearest the discharge site (P1) resulted in the most severe- and highest rates
of change in embryos and larvae, suggesting that DLC contaminated sediment probably
did not occur much beyond it. P1 extract resulted in concentration dependent increases
in mortality and pericardial edema. Its highest concentration caused up-regulation
of P-450 (CYP)-1A1(CYP1A) mRNA expression at 72 h post fertilization (hpf), an increase
in its expression in gill arches as observed by whole mount in situ hybridization,
and an increased signal in the Tg(cyp1a: mCherry) transgenic line. The pattern and
magnitude of response was very similar to that of TCDD and supported the presence
of DLCs in these sediment samples. Follow-up chemical analysis confirmed this presence
and identified H7CDF, O8CDF and O8CDD as the main components in P1 extract. This study
validates the use of biological assays as a rapid, sensitive, and cost-effective method
to evaluate DLCs and their effects in sediment samples. Additionally, it provides
support for the conclusion that DLCs have limited remobilization capacity in marine
sediments.
Type
Journal articleSubject
Embryo, NonmammalianAnimals
Zebrafish
Dioxins
Water Pollutants, Chemical
Environmental Monitoring
Embryonic Development
Geologic Sediments
China
Permalink
https://hdl.handle.net/10161/19204Published Version (Please cite this version)
10.1016/j.ecoenv.2019.01.116Publication Info
Dong, Wenjing; Wang, Feng; Fang, Mingliang; Wu, Jie; Wang, Shuaiyu; Li, Ming; ...
Dong, Wu (2019). Use of biological detection methods to assess dioxin-like compounds in sediments of
Bohai Bay, China. Ecotoxicology and environmental safety, 173. pp. 339-346. 10.1016/j.ecoenv.2019.01.116. Retrieved from https://hdl.handle.net/10161/19204.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
Collections
More Info
Show full item record
Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info