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Esterified Trehalose Analogues Protect Mammalian Cells from Heat Shock.
Abstract
Trehalose is a disaccharide produced by many organisms to better enable them to survive
environmental stresses, including heat, cold, desiccation, and reactive oxygen species.
Mammalian cells do not naturally biosynthesize trehalose; however, when introduced
into mammalian cells, trehalose provides protection from damage associated with freezing
and drying. One of the major difficulties in using trehalose as a cellular protectant
for mammalian cells is the delivery of this disaccharide into the intracellular environment;
mammalian cell membranes are impermeable to the hydrophilic sugar trehalose. A panel
of cell-permeable trehalose analogues, in which the hydrophilic hydroxyl groups of
trehalose are masked as esters, have been synthesized and the ability of these analogues
to load trehalose into mammalian cells has been evaluated. Two of these analogues
deliver millimolar concentrations of free trehalose into a variety of mammalian cells.
Critically, Jurkat cells incubated with these analogues show improved survival after
heat shock, relative to untreated Jurkat cells. The method reported herein thus paves
the way for the use of esterified analogues of trehalose as a facile means to deliver
high concentrations of trehalose into mammalian cells for use as a cellular protectant.
Type
Journal articleSubject
Hela CellsJurkat Cells
NIH 3T3 Cells
Animals
Humans
Mice
Trehalose
Temperature
Cell Survival
Esterification
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https://hdl.handle.net/10161/19694Published Version (Please cite this version)
10.1002/cbic.201700302Publication Info
Bragg, Jack T; D'Ambrosio, Hannah K; Smith, Timothy J; Gorka, Caroline A; Khan, Faraz
A; Rose, Joshua T; ... Paulick, Margot G (2017). Esterified Trehalose Analogues Protect Mammalian Cells from Heat Shock. Chembiochem : a European journal of chemical biology, 18(18). pp. 1863-1870. 10.1002/cbic.201700302. Retrieved from https://hdl.handle.net/10161/19694.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Michael Scott Boyce
Associate Professor of Biochemistry
The Boyce Lab studies mammalian cell signaling through protein glycosylation. For
the latest news, project information and publications from our group, please visit
our web site at http://www.boycelab.org or follow us on Twitter at https://twitter.com/BoyceLab.
Tim Smith
Student
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