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Esterified Trehalose Analogues Protect Mammalian Cells from Heat Shock.

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Date
2017-09
Authors
Bragg, Jack T
D'Ambrosio, Hannah K
Smith, Timothy J
Gorka, Caroline A
Khan, Faraz A
Rose, Joshua T
Rouff, Andrew J
Fu, Terence S
Bisnett, Brittany J
Boyce, Michael
Khetan, Sudhir
Paulick, Margot G
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(12 total)
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Abstract
Trehalose is a disaccharide produced by many organisms to better enable them to survive environmental stresses, including heat, cold, desiccation, and reactive oxygen species. Mammalian cells do not naturally biosynthesize trehalose; however, when introduced into mammalian cells, trehalose provides protection from damage associated with freezing and drying. One of the major difficulties in using trehalose as a cellular protectant for mammalian cells is the delivery of this disaccharide into the intracellular environment; mammalian cell membranes are impermeable to the hydrophilic sugar trehalose. A panel of cell-permeable trehalose analogues, in which the hydrophilic hydroxyl groups of trehalose are masked as esters, have been synthesized and the ability of these analogues to load trehalose into mammalian cells has been evaluated. Two of these analogues deliver millimolar concentrations of free trehalose into a variety of mammalian cells. Critically, Jurkat cells incubated with these analogues show improved survival after heat shock, relative to untreated Jurkat cells. The method reported herein thus paves the way for the use of esterified analogues of trehalose as a facile means to deliver high concentrations of trehalose into mammalian cells for use as a cellular protectant.
Type
Journal article
Subject
Hela Cells
Jurkat Cells
NIH 3T3 Cells
Animals
Humans
Mice
Trehalose
Temperature
Cell Survival
Esterification
Permalink
https://hdl.handle.net/10161/19694
Published Version (Please cite this version)
10.1002/cbic.201700302
Publication Info
Bragg, Jack T; D'Ambrosio, Hannah K; Smith, Timothy J; Gorka, Caroline A; Khan, Faraz A; Rose, Joshua T; ... Paulick, Margot G (2017). Esterified Trehalose Analogues Protect Mammalian Cells from Heat Shock. Chembiochem : a European journal of chemical biology, 18(18). pp. 1863-1870. 10.1002/cbic.201700302. Retrieved from https://hdl.handle.net/10161/19694.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Boyce

Michael Scott Boyce

Associate Professor of Biochemistry
The Boyce Lab studies mammalian cell signaling through protein glycosylation. For the latest news, project information and publications from our group, please visit our web site at http://www.boycelab.org or follow us on Twitter at https://twitter.com/BoyceLab.
Smith

Tim Smith

Student
Alphabetical list of authors with Scholars@Duke profiles.
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