Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following HIV Envelope Immunization.
Abstract
HIV-1-infected infants develop broadly neutralizing antibodies (bnAbs) more rapidly
than adults, suggesting differences in the neonatal versus adult responses to the
HIV-1 envelope (Env). Here, trimeric forms of HIV-1 Env immunogens elicit increased
gp120- and gp41-specific antibodies more rapidly in neonatal macaques than adult macaques.
Transcriptome analyses of neonatal versus adult immune cells after Env vaccination
reveal that neonatal macaques have higher levels of the apoptosis regulator BCL2 in
T cells and lower levels of the immunosuppressive interleukin-10 (IL-10) receptor
alpha (IL10RA) mRNA transcripts in T cells, B cells, natural killer (NK) cells, and
monocytes. In addition, immunized neonatal macaques exhibit increased frequencies
of activated blood T follicular helper-like (Tfh) cells compared to adults. Thus,
neonatal macaques have transcriptome signatures of decreased immunosuppression and
apoptosis compared with adult macaques, providing an immune landscape conducive to
early-life immunization prior to sexual debut.
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https://hdl.handle.net/10161/20297Published Version (Please cite this version)
10.1016/j.celrep.2019.12.091Publication Info
(2020). Neonatal Rhesus Macaques Have Distinct Immune Cell Transcriptional Profiles following
HIV Envelope Immunization. Cell reports, 30(5). pp. 1553-1569.e6. 10.1016/j.celrep.2019.12.091. Retrieved from https://hdl.handle.net/10161/20297.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Derek Wilson Cain
Assistant Professor in Medicine
My research focuses on the interactions of T cells and B cells during infection or
following vaccination. I am particularly interested in the inter- and intracellular
events that take place within germinal centers, the anatomic site of antibody evolution
during an immune response.
Guido Ferrari
Associate Professor of Surgery
The activities of the Ferrari Laboratory are based on both independent basic research
and immune monitoring studies. The research revolves around three main areas of interest:
class I-mediated cytotoxic CD8+ T cell responses, antibody-dependent cellular cytotoxicity
(ADCC), gene expression in NK and T cellular subsets upon infection with HIV-1. With
continuous funding over the last 11 years from the NIH and Bill & Melinda Gates Foundation
along with many other productive collaborations wi
Barton Ford Haynes
Frederic M. Hanes Distinguished Professor of Medicine
The Haynes lab is studying host innate and adaptive immune responses to the human
immunodeficiency virus (HIV), tuberculosis (TB), and influenza in order to find the
enabling technology to make preventive vaccines against these three major infectious
diseases. Mucosal Immune Responses in Acute HIV Infection The Haynes lab is working
to determine why broadly neutralizing antibodies are rarely made in acute HIV infection
(AHI), currently a major obstacle in the de
David Charles Montefiori
Professor of Surgery
Dr. Montefiori is Professor and Director of the Laboratory for AIDS Vaccine Research
and Development in the Department of Surgery, Division of Surgical Sciences, Duke
University Medical Center. His major research interests are viral immunology and AIDS
vaccine development, with a special emphasis on neutralizing antibodies. One of his
highest priorities is to identify immunogens that generate broadly cross-reactive
neutralizing antibodies for inclusion in HIV vaccines. Many aspects of the
Michael Anthony Moody
Associate Professor of Pediatrics
Tony Moody, MD is an Associate Professor in the Department of Pediatrics, Division
of Infectious Diseases and the Department of Immunology at Duke University Medical
Center. Research in the Moody lab is focused on understanding the B cell responses
during infection, vaccination, and disease. The lab has become a resource for human
phenotyping, flow characterization, staining and analysis at the Duke Human Vaccine
Institute (DHVI). The Moody lab is currently funded to study influenza, syphilis
Sallie Robey Permar
Wilburt C. Davison Distinguished Professor
Dr. Permar's work focuses on the development of vaccines to prevent vertical transmission
of neonatal viral pathogens. She has utilized the nonhuman primate model of HIV/AIDS
to characterize the virus-specific immune responses and virus evolution in breast
milk and develop a maternal vaccine regimen for protection against breast milk transmission
of HIV. In addition, Dr. Permar's lab has advanced the understanding of HIV-specific
immune responses and virus evolution in vertically-transmitting an
Kevin O'Neil Saunders
Associate Professor in Surgery
The Saunders laboratory aims to understand the immunology of HIV-1 antibodies and
the molecular biology of their interaction with HIV-1 envelope (Env) glycoprotein.
Our overall goal is to develop protective antibody-based vaccines; therefore, the
laboratory has two sections–antibody repertoire analysis and immunogen design.
Our research premise is that vaccine-elicited antibodies will broadly neutralize HIV-1
if they can bind directly to the host glycans on Env. However, Env glycans are
Xiaoying Shen
Associate Professor in Surgery
Georgia Doris Tomaras
Professor in Surgery
Research in the Tomaras Laboratory in the Duke Human Vaccine Institute and Departments
of Surgery, Immunology, and Molecular Genetics and Microbiology at Duke University
Medical Center, focuses on the identification of immune correlates of protection for
preventative vaccines and identification of the mechanisms responsible for potent
inhibition of human pathogens.
Kevin J Wiehe
Associate Professor in Medicine
Wilton Bryan Williams
Assistant Professor in Medicine
Dr. Williams completed a PhD in Biomedical Sciences (Immunology and Microbiology)
from the University of Florida and did his postdoctoral work in the laboratory of
Dr. Barton Haynes at the Duke Human Vaccine Institute (DHVI). The key goals of HIV
vaccine development are to define the host-virus events during natural HIV infection
that lead to the induction of broadly neutralizing antibodies, and to recreate those
events with a vaccine. As a junior faculty member in the DHVI, Dr. W
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