Regulation of Integrin α6 Recycling by Calcium-independent Phospholipase A2 (iPLA2) to Promote Microglia Chemotaxis on Laminin.
Abstract
Microglia are the immune effector cells that are activated in response to pathological
changes in the central nervous system. Microglial activation is accompanied by the
alteration of integrin expression on the microglia surface. However, changes of integrin
expression upon chemoattractant (ADP) stimulation still remain unknown. In this study,
we investigated whether ADP induces the alteration of integrin species on the cell
surface, leading to changes in chemotactic ability on different extracellular matrix
proteins. Flow cytometry scans and on-cell Western assays showed that ADP stimulation
induced a significant increase of α6 integrin-GFP, but not α5, on the surface of microglia
cells. Microglia also showed a greater motility increase on laminin than fibronectin
after ADP stimulation. Time lapse microscopy and integrin endocytosis assay revealed
the essential role of calcium-independent phospholipase A2 activity for the recycling
of α6 integrin-GFP from the endosomal recycling complex to the plasma membrane. Lack
of calcium-independent phospholipase A2 activity caused a reduced rate of focal adhesion
formation on laminin at the leading edge. Our results suggest that the alteration
of integrin-mediated adhesion may regulate the extent of microglial infiltration into
the site of damage by controlling their chemotactic ability.
Type
Journal articleSubject
MicrogliaCell Line
Animals
Humans
Mice
Laminin
Integrin alpha6
RNA, Small Interfering
Adenosine Diphosphate
Cell Movement
Chemotaxis
RNA Interference
Protein Transport
Phospholipases A2, Calcium-Independent
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https://hdl.handle.net/10161/21062Published Version (Please cite this version)
10.1074/jbc.m116.732610Publication Info
Lee, Sang-Hyun; Sud, Neetu; Lee, Narae; Subramaniyam, Selvaraj; & Chung, Chang Y (2016). Regulation of Integrin α6 Recycling by Calcium-independent Phospholipase A2 (iPLA2)
to Promote Microglia Chemotaxis on Laminin. The Journal of biological chemistry, 291(45). pp. 23645-23653. 10.1074/jbc.m116.732610. Retrieved from https://hdl.handle.net/10161/21062.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Chang Yong Chung
Professor of Biology at Duke Kunshan University

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