MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management.
Abstract
Negative Pressure Wound Therapy (NPWT), a widely used modality in the management of
surgical and trauma wounds, offers clear benefits over conventional wound healing
strategies. Despite the wide-ranging effects ascribed to NPWT, the precise molecular
mechanisms underlying the accelerated healing supported by NPWT remains poorly understood.
Notably, cellular redox status-a product of the balance between cellular reactive
oxygen species (ROS) production and anti-oxidant defense systems-plays an important
role in wound healing and dysregulation of redox homeostasis has a profound effect
on wound healing. Here we investigated potential links between the use of NPWT and
the regulation of antioxidant mechanisms. Using patient samples and a rodent model
of acute injury, we observed a significant accumulation of MnSOD protein as well as
higher enzymatic activity in tissues upon NPWT. As a proof of concept and to outline
the important role of SOD activity in wound healing, we replaced NPWT by the topical
application of a MnSOD mimetic, Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin
(MnTE-2-PyP5+, MnE, BMX-010, AEOl10113) in the rodent model. We observed that MnE
is a potent wound healing enhancer as it appears to facilitate the formation of new
tissue within the wound bed and consequently advances wound closure by two days, compared
to the non-treated animals. Taken together, these results show for the first time
a link between NPWT and regulation of antioxidant mechanism through the maintenance
of MnSOD activity. Additionally this discovery outlined the potential role of MnSOD
mimetics as topical agents enhancing wound healing.
Type
Journal articleSubject
AnimalsHumans
Rats
Disease Models, Animal
Metalloporphyrins
Superoxide Dismutase
Antioxidants
Treatment Outcome
Combined Modality Therapy
Administration, Topical
Biomimetics
Wound Healing
Enzyme Activation
Disease Management
Negative-Pressure Wound Therapy
Biological Mimicry
Permalink
https://hdl.handle.net/10161/21189Published Version (Please cite this version)
10.1016/j.redox.2018.10.014Publication Info
Bellot, Gregory Lucien; Dong, Xiaoke; Lahiri, Amitabha; Sebastin, Sandeep Jacob; Batinic-Haberle,
Ines; Pervaiz, Shazib; & Puhaindran, Mark Edward (2019). MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy
(NPWT): A case in point for MnSOD mimetics as adjuvants for wound management. Redox biology, 20. pp. 307-320. 10.1016/j.redox.2018.10.014. Retrieved from https://hdl.handle.net/10161/21189.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
Collections
More Info
Show full item recordScholars@Duke
Ines Batinic-Haberle
Professor Emeritus of Radiation Oncology
A major interest of mine has been in the design and synthesis of Mn porphyrin(MnP)-based
powerful catalytic antioxidants which helped establish structure-activity relationship
(SAR). It relates the redox property of metalloporphyrins to their ability to remove
superoxide. SAR has facilitated the design of redox-active therapeutics and served
as a tool for mechanistic considerations. Importantly SAR parallels the magnitu

Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info
Related items
Showing items related by title, author, creator, and subject.
-
Ballistic Wounding
Severance, Harry (Critical Decisions in Emergency Medicine, 1999-03-01) -
Closed-Incision Negative-Pressure Therapy in Obese Patients Undergoing Cesarean Delivery: A Randomized Controlled Trial.
Gunatilake, Ravindu P; Swamy, Geeta K; Brancazio, Leo R; Smrtka, Michael P; Thompson, Jennifer L; Gilner, Jennifer B; Gray, Beverly A; ... (8 authors) (AJP Rep, 2017-07)Background Postcesarean wound morbidity is a costly complication of cesarean delivery for which preventative strategies remain understudied.Objective We compared surgical site occurrences (SSOs) in cesarean patients receiving ... -
Exploring the Interface Between Macroorganisms and Microorganisms: Biochemical, Ecological, and Evolutionary Contexts
Essock-Burns, Tara (2015)The focus of this dissertation is the extension of the innate immune response in wound healing and non-wound healing contexts. I am interested in interactions at the interface between macroorganisms and microorganisms from ...