A plant genetic network for preventing dysbiosis in the phyllosphere.
Abstract
The aboveground parts of terrestrial plants, collectively called the phyllosphere,
have a key role in the global balance of atmospheric carbon dioxide and oxygen. The
phyllosphere represents one of the most abundant habitats for microbiota colonization.
Whether and how plants control phyllosphere microbiota to ensure plant health is not
well understood. Here we show that the Arabidopsis quadruple mutant (min7 fls2 efr
cerk1; hereafter, mfec)1, simultaneously defective in pattern-triggered immunity and
the MIN7 vesicle-trafficking pathway, or a constitutively activated cell death1 (cad1)
mutant, carrying a S205F mutation in a membrane-attack-complex/perforin (MACPF)-domain
protein, harbour altered endophytic phyllosphere microbiota and display leaf-tissue
damage associated with dysbiosis. The Shannon diversity index and the relative abundance
of Firmicutes were markedly reduced, whereas Proteobacteria were enriched in the mfec
and cad1S205F mutants, bearing cross-kingdom resemblance to some aspects of the dysbiosis
that occurs in human inflammatory bowel disease. Bacterial community transplantation
experiments demonstrated a causal role of a properly assembled leaf bacterial community
in phyllosphere health. Pattern-triggered immune signalling, MIN7 and CAD1 are found
in major land plant lineages and are probably key components of a genetic network
through which terrestrial plants control the level and nurture the diversity of endophytic
phyllosphere microbiota for survival and health in a microorganism-rich environment.
Type
Journal articleSubject
ProteobacteriaArabidopsis
Plant Components, Aerial
Plant Leaves
Guanine Nucleotide Exchange Factors
Arabidopsis Proteins
Environment
Cell Death
Plant Diseases
Homeostasis
Genotype
Phenotype
Mutation
Genes, Plant
Gene Regulatory Networks
Plant Immunity
Microbiota
Firmicutes
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https://hdl.handle.net/10161/21718Published Version (Please cite this version)
10.1038/s41586-020-2185-0Publication Info
Chen, Tao; Nomura, Kinya; Wang, Xiaolin; Sohrabi, Reza; Xu, Jin; Yao, Lingya; ...
He, Sheng Yang (2020). A plant genetic network for preventing dysbiosis in the phyllosphere. Nature, 580(7805). pp. 653-657. 10.1038/s41586-020-2185-0. Retrieved from https://hdl.handle.net/10161/21718.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Sheng-Yang He
Benjamin E. Powell Distinguished Professor of Biology
Interested in the fascinating world of plants, microbes or inter-organismal communication
and co-evolution? Please contact Prof. Sheng-Yang He (shengyang.he@duke.edu; hes@msu.edu).
Millions of years of co-evolution between plants and microbes have resulted in an
intricate web of attack, counter-attack, decoy, and hijacking mechanisms in biology.
Moreover, co-evolution between plants and microbes is greatly impacted by ongoing
climate

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